Untargeted Liquid Chromatography–High-Resolution Mass Spectrometry Metabolomic Investigation Reveals Altered Lipid Content in <i>Leishmania infantum</i> Lacking Lipid Droplet Protein Kinase
Leishmaniasis is a complex disease caused by different species of <i>Leishmania</i>. To date, no vaccine for humans or ideal therapy has been developed owing to the limited efficacy and toxicity of available drugs, as well as the emergence of resistant strains. Therefore, it is necessary...
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2024-09-01
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| author | Juliana Martins Ribeiro Gisele André Baptista Canuto Alisson Samuel Portes Caldeira Ezequias Pessoa de Siqueira Carlos Leomar Zani Silvane Maria Fonseca Murta Tânia Maria de Almeida Alves |
| author_facet | Juliana Martins Ribeiro Gisele André Baptista Canuto Alisson Samuel Portes Caldeira Ezequias Pessoa de Siqueira Carlos Leomar Zani Silvane Maria Fonseca Murta Tânia Maria de Almeida Alves |
| author_sort | Juliana Martins Ribeiro |
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| description | Leishmaniasis is a complex disease caused by different species of <i>Leishmania</i>. To date, no vaccine for humans or ideal therapy has been developed owing to the limited efficacy and toxicity of available drugs, as well as the emergence of resistant strains. Therefore, it is necessary to identify novel therapeutic targets and discover therapeutic options for leishmaniasis. In this study, we evaluated the impact of deleting the lipid droplet protein kinase (LDK) enzyme in <i>Leishmania infantum</i> using an untargeted metabolomics approach performed using liquid chromatography and high-resolution mass spectrometry. LDK is involved in lipid droplet biogenesis in trypanosomatids. Thirty-nine lipid metabolites altered in the stationary and logarithmic growth phases were noted and classified into five classes: (1) sterols, (2) fatty and conjugated acids, (3) ceramides, (4) glycerophosphocholine and its derivatives, and (5) glycerophosphoethanolamine and its derivatives. Our data demonstrated that glycerophosphocholine and its derivatives were the most affected after LDK deletion, suggesting that the absence of this enzyme promotes the remodeling of lipid composition in <i>L. infantum</i>, thus contributing to a better understanding of the function of LDK in this parasite. |
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| issn | 2414-6366 |
| language | English |
| publishDate | 2024-09-01 |
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| series | Tropical Medicine and Infectious Disease |
| spelling | doaj-art-85f915b7fd08438e9ac8dcd099ff92b02025-08-20T01:55:53ZengMDPI AGTropical Medicine and Infectious Disease2414-63662024-09-019920810.3390/tropicalmed9090208Untargeted Liquid Chromatography–High-Resolution Mass Spectrometry Metabolomic Investigation Reveals Altered Lipid Content in <i>Leishmania infantum</i> Lacking Lipid Droplet Protein KinaseJuliana Martins Ribeiro0Gisele André Baptista Canuto1Alisson Samuel Portes Caldeira2Ezequias Pessoa de Siqueira3Carlos Leomar Zani4Silvane Maria Fonseca Murta5Tânia Maria de Almeida Alves6Grupo Genômica Funcional de Parasitos, Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte 30190-002, Minas Gerais, BrazilDepartamento de Química Analítica, Instituto de Química, Universidade Federal da Bahia, Salvador 40170-115, Bahia, BrazilGrupo Química de Produtos Naturais Bioativos, Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte 30190-002, Minas Gerais, BrazilGrupo Química de Produtos Naturais Bioativos, Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte 30190-002, Minas Gerais, BrazilGrupo Química de Produtos Naturais Bioativos, Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte 30190-002, Minas Gerais, BrazilGrupo Genômica Funcional de Parasitos, Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte 30190-002, Minas Gerais, BrazilGrupo Química de Produtos Naturais Bioativos, Instituto René Rachou, Fundação Oswaldo Cruz, Belo Horizonte 30190-002, Minas Gerais, BrazilLeishmaniasis is a complex disease caused by different species of <i>Leishmania</i>. To date, no vaccine for humans or ideal therapy has been developed owing to the limited efficacy and toxicity of available drugs, as well as the emergence of resistant strains. Therefore, it is necessary to identify novel therapeutic targets and discover therapeutic options for leishmaniasis. In this study, we evaluated the impact of deleting the lipid droplet protein kinase (LDK) enzyme in <i>Leishmania infantum</i> using an untargeted metabolomics approach performed using liquid chromatography and high-resolution mass spectrometry. LDK is involved in lipid droplet biogenesis in trypanosomatids. Thirty-nine lipid metabolites altered in the stationary and logarithmic growth phases were noted and classified into five classes: (1) sterols, (2) fatty and conjugated acids, (3) ceramides, (4) glycerophosphocholine and its derivatives, and (5) glycerophosphoethanolamine and its derivatives. Our data demonstrated that glycerophosphocholine and its derivatives were the most affected after LDK deletion, suggesting that the absence of this enzyme promotes the remodeling of lipid composition in <i>L. infantum</i>, thus contributing to a better understanding of the function of LDK in this parasite.https://www.mdpi.com/2414-6366/9/9/208lipid droplet protein kinase<i>L. infantum</i>untargeted metabolomicsliquid chromatography–high-resolution mass spectrometry (LC-HRMS) |
| spellingShingle | Juliana Martins Ribeiro Gisele André Baptista Canuto Alisson Samuel Portes Caldeira Ezequias Pessoa de Siqueira Carlos Leomar Zani Silvane Maria Fonseca Murta Tânia Maria de Almeida Alves Untargeted Liquid Chromatography–High-Resolution Mass Spectrometry Metabolomic Investigation Reveals Altered Lipid Content in <i>Leishmania infantum</i> Lacking Lipid Droplet Protein Kinase Tropical Medicine and Infectious Disease lipid droplet protein kinase <i>L. infantum</i> untargeted metabolomics liquid chromatography–high-resolution mass spectrometry (LC-HRMS) |
| title | Untargeted Liquid Chromatography–High-Resolution Mass Spectrometry Metabolomic Investigation Reveals Altered Lipid Content in <i>Leishmania infantum</i> Lacking Lipid Droplet Protein Kinase |
| title_full | Untargeted Liquid Chromatography–High-Resolution Mass Spectrometry Metabolomic Investigation Reveals Altered Lipid Content in <i>Leishmania infantum</i> Lacking Lipid Droplet Protein Kinase |
| title_fullStr | Untargeted Liquid Chromatography–High-Resolution Mass Spectrometry Metabolomic Investigation Reveals Altered Lipid Content in <i>Leishmania infantum</i> Lacking Lipid Droplet Protein Kinase |
| title_full_unstemmed | Untargeted Liquid Chromatography–High-Resolution Mass Spectrometry Metabolomic Investigation Reveals Altered Lipid Content in <i>Leishmania infantum</i> Lacking Lipid Droplet Protein Kinase |
| title_short | Untargeted Liquid Chromatography–High-Resolution Mass Spectrometry Metabolomic Investigation Reveals Altered Lipid Content in <i>Leishmania infantum</i> Lacking Lipid Droplet Protein Kinase |
| title_sort | untargeted liquid chromatography high resolution mass spectrometry metabolomic investigation reveals altered lipid content in i leishmania infantum i lacking lipid droplet protein kinase |
| topic | lipid droplet protein kinase <i>L. infantum</i> untargeted metabolomics liquid chromatography–high-resolution mass spectrometry (LC-HRMS) |
| url | https://www.mdpi.com/2414-6366/9/9/208 |
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