Simultaneous quantification of 12 water-soluble vitamins in MDCK cell culture media by eco-friendly ion-pairing reagent-free HPLC-UV

Simultaneous quantification of 12 water-soluble vitamins in MDCK cell culture media by eco-friendly ion-pairing reagent-free HPLC-UV

Abstract This study establishes the first reversed-phase HPLC-UV method for simultaneous baseline separation of 12 structurally diverse water-soluble vitamins (WSVs) within 25 min. The eco-friendly approach utilizes a methanol–potassium dihydrogen phosphate (pH 4.85) gradient on a ChromCore C18 colu...

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Bibliographic Details
Main Authors: Lei Zhou, Mengting Zhang, Huinan Sun, Liyuan Duan, Qingwei Meng
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
Subjects:
HPLC-UV
Water-soluble vitamins
Green analytical chemistry
MDCK cell culture media
Simultaneous quantification
Online Access:https://doi.org/10.1038/s41598-025-12536-z
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https://doi.org/10.1038/s41598-025-12536-z

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