Impairment of theta oscillations in the hippocampal CA1 region may mediate age-dependent movement alternations in the 5xFAD mouse model of Alzheimer’s disease

Abstract Clinical evidences indicate that multifaceted gait abnormalities may manifest in Alzheimer’s disease (AD) patients, which are associated with cognitive decline. Although the correlation between hippocampal theta power and locomotion has been known for a long time, the mechanisms by how hipp...

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Main Authors: Hong Ni, Zhongzhao Guo, Jie Wang, Zilu Zhu, Chenyi Xia, Ming Xu, Guohui Zhang, Deheng Wang
Format: Article
Language:English
Published: Nature Portfolio 2025-03-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-95585-8
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author Hong Ni
Zhongzhao Guo
Jie Wang
Zilu Zhu
Chenyi Xia
Ming Xu
Guohui Zhang
Deheng Wang
author_facet Hong Ni
Zhongzhao Guo
Jie Wang
Zilu Zhu
Chenyi Xia
Ming Xu
Guohui Zhang
Deheng Wang
author_sort Hong Ni
collection DOAJ
description Abstract Clinical evidences indicate that multifaceted gait abnormalities may manifest in Alzheimer’s disease (AD) patients, which are associated with cognitive decline. Although the correlation between hippocampal theta power and locomotion has been known for a long time, the mechanisms by how hippocampal impairment participates in the altered gait seen in AD is not fully understood. To explore the manifestations of gait disorders in AD, we characterized gait performance in 3-, 6-, and 9-month-old male 5xFAD and control mice in the semi-automated, highly sensitive, Catwalk XT system. The 5xFAD mice displayed a decrease in kinetic parameters (average speed and cadence), and spatial parameters (paw area), while the temporal parameters (stance and swing time) were significantly increased. The parameters of interlimb coordination also displayed deficits. The majority of impairment variables related to the slow speed in 5xFAD mice at 9-month-old. We further explored the theta oscillations in the brain by in vivo tetrode recording of the hippocampal CA1. The results showed that the theta oscillations reduced in the hippocampal CA1 of 5xFAD mice, which related to the gait impairments. In conclusion, gait impairments started at 6 months of age, manifested at 9 months of age in 5xFAD mice. A reduction in theta oscillation power of the hippocampal CA1 may be responsible for the gait impairments.
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spelling doaj-art-8592d799bc5c4e2788d14b1c5829f65c2025-08-20T02:25:35ZengNature PortfolioScientific Reports2045-23222025-03-0115111810.1038/s41598-025-95585-8Impairment of theta oscillations in the hippocampal CA1 region may mediate age-dependent movement alternations in the 5xFAD mouse model of Alzheimer’s diseaseHong Ni0Zhongzhao Guo1Jie Wang2Zilu Zhu3Chenyi Xia4Ming Xu5Guohui Zhang6Deheng Wang7School of Integrative Medicine, Shanghai University of Traditional Chinese MedicineSchool of Integrative Medicine, Shanghai University of Traditional Chinese MedicineDepartment of Chinese Medicine & Integrative Medicine, Shanghai Geriatric Medical Center, Zhongshan Hospital, Fudan UniversitySchool of Integrative Medicine, Shanghai University of Traditional Chinese MedicineSchool of Integrative Medicine, Shanghai University of Traditional Chinese MedicineSchool of Integrative Medicine, Shanghai University of Traditional Chinese MedicineRehabilitation department, Yueyang Hospital of Integrated Traditional Chinese and Western MedicineSchool of Integrative Medicine, Shanghai University of Traditional Chinese MedicineAbstract Clinical evidences indicate that multifaceted gait abnormalities may manifest in Alzheimer’s disease (AD) patients, which are associated with cognitive decline. Although the correlation between hippocampal theta power and locomotion has been known for a long time, the mechanisms by how hippocampal impairment participates in the altered gait seen in AD is not fully understood. To explore the manifestations of gait disorders in AD, we characterized gait performance in 3-, 6-, and 9-month-old male 5xFAD and control mice in the semi-automated, highly sensitive, Catwalk XT system. The 5xFAD mice displayed a decrease in kinetic parameters (average speed and cadence), and spatial parameters (paw area), while the temporal parameters (stance and swing time) were significantly increased. The parameters of interlimb coordination also displayed deficits. The majority of impairment variables related to the slow speed in 5xFAD mice at 9-month-old. We further explored the theta oscillations in the brain by in vivo tetrode recording of the hippocampal CA1. The results showed that the theta oscillations reduced in the hippocampal CA1 of 5xFAD mice, which related to the gait impairments. In conclusion, gait impairments started at 6 months of age, manifested at 9 months of age in 5xFAD mice. A reduction in theta oscillation power of the hippocampal CA1 may be responsible for the gait impairments.https://doi.org/10.1038/s41598-025-95585-8Alzheimer’s disease5xFAD mouseGaitHippocampusTheta oscillations
spellingShingle Hong Ni
Zhongzhao Guo
Jie Wang
Zilu Zhu
Chenyi Xia
Ming Xu
Guohui Zhang
Deheng Wang
Impairment of theta oscillations in the hippocampal CA1 region may mediate age-dependent movement alternations in the 5xFAD mouse model of Alzheimer’s disease
Scientific Reports
Alzheimer’s disease
5xFAD mouse
Gait
Hippocampus
Theta oscillations
title Impairment of theta oscillations in the hippocampal CA1 region may mediate age-dependent movement alternations in the 5xFAD mouse model of Alzheimer’s disease
title_full Impairment of theta oscillations in the hippocampal CA1 region may mediate age-dependent movement alternations in the 5xFAD mouse model of Alzheimer’s disease
title_fullStr Impairment of theta oscillations in the hippocampal CA1 region may mediate age-dependent movement alternations in the 5xFAD mouse model of Alzheimer’s disease
title_full_unstemmed Impairment of theta oscillations in the hippocampal CA1 region may mediate age-dependent movement alternations in the 5xFAD mouse model of Alzheimer’s disease
title_short Impairment of theta oscillations in the hippocampal CA1 region may mediate age-dependent movement alternations in the 5xFAD mouse model of Alzheimer’s disease
title_sort impairment of theta oscillations in the hippocampal ca1 region may mediate age dependent movement alternations in the 5xfad mouse model of alzheimer s disease
topic Alzheimer’s disease
5xFAD mouse
Gait
Hippocampus
Theta oscillations
url https://doi.org/10.1038/s41598-025-95585-8
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