Calcium levels modulate embryo yield in Brassica napus microspore embryogenesis
Calcium (Ca2+) is a universal signaling cation with a prominent role as second messenger in many different plant processes, including sexual reproduction. However, there is much less knowledge about the involvement of Ca2+ during in vitro embryogenesis processes. In this work we performed a study of...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1512500/full |
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| author | Antonio Calabuig-Serna Ricardo Mir Daniel Sancho-Oviedo Paloma Arjona-Mudarra Jose M. Seguí-Simarro |
| author_facet | Antonio Calabuig-Serna Ricardo Mir Daniel Sancho-Oviedo Paloma Arjona-Mudarra Jose M. Seguí-Simarro |
| author_sort | Antonio Calabuig-Serna |
| collection | DOAJ |
| description | Calcium (Ca2+) is a universal signaling cation with a prominent role as second messenger in many different plant processes, including sexual reproduction. However, there is much less knowledge about the involvement of Ca2+ during in vitro embryogenesis processes. In this work we performed a study of Ca2+ levels during the different stages of microspore embryogenesis in Brassica napus, with special attention to how Ca2+ can influence the occurrence of different embryogenic structures with different embryogenic potential. We also performed a pharmacological study to modulate Ca2+ homeostasis during different stages of the process, using a series of Ca2+-altering chemicals (BAPTA-AM, bepridil, chlorpromazine, cyclopiazonic acid, EGTA, inositol 1,4,5-trisphosphate, ionophore A23187, W-7). This study shows that Ca2+ increase can be considered as an early marker of induction of microspore embryogenesis. Besides, Ca2+ levels are highly dynamic during microspore embryogenesis, influencing the final embryo yield. Increase of either extracellular or intracellular Ca2+ levels improves embryo yield without altering the proportion of highly embryogenic structures formed, which suggests that elevated Ca2+ levels increase the amount of microspores reaching the minimum Ca2+ threshold required for embryogenesis induction. Conversely, inhibition of Ca2+ uptake or signaling results in reduced embryogenic response. This allows to modulate embryo yield within a functional range, with lower and upper Ca2+ thresholds beyond which embryo yield is reduced. There seems to be a relationship between Ca2+ levels and embryo differentiation. |
| format | Article |
| id | doaj-art-858dad889ef145ed92c2b3e6cdd64dcf |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-858dad889ef145ed92c2b3e6cdd64dcf2025-01-16T06:10:32ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.15125001512500Calcium levels modulate embryo yield in Brassica napus microspore embryogenesisAntonio Calabuig-SernaRicardo MirDaniel Sancho-OviedoPaloma Arjona-MudarraJose M. Seguí-SimarroCalcium (Ca2+) is a universal signaling cation with a prominent role as second messenger in many different plant processes, including sexual reproduction. However, there is much less knowledge about the involvement of Ca2+ during in vitro embryogenesis processes. In this work we performed a study of Ca2+ levels during the different stages of microspore embryogenesis in Brassica napus, with special attention to how Ca2+ can influence the occurrence of different embryogenic structures with different embryogenic potential. We also performed a pharmacological study to modulate Ca2+ homeostasis during different stages of the process, using a series of Ca2+-altering chemicals (BAPTA-AM, bepridil, chlorpromazine, cyclopiazonic acid, EGTA, inositol 1,4,5-trisphosphate, ionophore A23187, W-7). This study shows that Ca2+ increase can be considered as an early marker of induction of microspore embryogenesis. Besides, Ca2+ levels are highly dynamic during microspore embryogenesis, influencing the final embryo yield. Increase of either extracellular or intracellular Ca2+ levels improves embryo yield without altering the proportion of highly embryogenic structures formed, which suggests that elevated Ca2+ levels increase the amount of microspores reaching the minimum Ca2+ threshold required for embryogenesis induction. Conversely, inhibition of Ca2+ uptake or signaling results in reduced embryogenic response. This allows to modulate embryo yield within a functional range, with lower and upper Ca2+ thresholds beyond which embryo yield is reduced. There seems to be a relationship between Ca2+ levels and embryo differentiation.https://www.frontiersin.org/articles/10.3389/fpls.2024.1512500/fullandrogenesisCa2+in vitro culturein vitro embryogenesismorphogenesisdoubled haploids |
| spellingShingle | Antonio Calabuig-Serna Ricardo Mir Daniel Sancho-Oviedo Paloma Arjona-Mudarra Jose M. Seguí-Simarro Calcium levels modulate embryo yield in Brassica napus microspore embryogenesis Frontiers in Plant Science androgenesis Ca2+ in vitro culture in vitro embryogenesis morphogenesis doubled haploids |
| title | Calcium levels modulate embryo yield in Brassica napus microspore embryogenesis |
| title_full | Calcium levels modulate embryo yield in Brassica napus microspore embryogenesis |
| title_fullStr | Calcium levels modulate embryo yield in Brassica napus microspore embryogenesis |
| title_full_unstemmed | Calcium levels modulate embryo yield in Brassica napus microspore embryogenesis |
| title_short | Calcium levels modulate embryo yield in Brassica napus microspore embryogenesis |
| title_sort | calcium levels modulate embryo yield in brassica napus microspore embryogenesis |
| topic | androgenesis Ca2+ in vitro culture in vitro embryogenesis morphogenesis doubled haploids |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1512500/full |
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