Viral diversity in wild rodents in the regions of Canaã de Carajás and Curionopólis, State of Pará, Brazil
Wild rodents serve as crucial reservoirs for zoonotic viruses. Anthropogenic and environmental disruptions, particularly those induced by mining activities, can destabilize rodent populations and facilitate the emergence of viral agents. In the Canaã dos Carajás and Curionópolis regions of Brazil, s...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1502462/full |
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| author | Adriana Freitas Moraes Monteiro Adriana Freitas Moraes Monteiro Fábio Silva da Silva Ana Cecília Ribeiro Cruz Ana Cecília Ribeiro Cruz Sandro Patroca da Silva Alice Louize Nunes Queiroz Livia Medeiros Neves Casseb Livia Medeiros Neves Casseb Livia Carício Martins Livia Carício Martins Daniele Barbosa de Almeida Medeiros Daniele Barbosa de Almeida Medeiros |
| author_facet | Adriana Freitas Moraes Monteiro Adriana Freitas Moraes Monteiro Fábio Silva da Silva Ana Cecília Ribeiro Cruz Ana Cecília Ribeiro Cruz Sandro Patroca da Silva Alice Louize Nunes Queiroz Livia Medeiros Neves Casseb Livia Medeiros Neves Casseb Livia Carício Martins Livia Carício Martins Daniele Barbosa de Almeida Medeiros Daniele Barbosa de Almeida Medeiros |
| author_sort | Adriana Freitas Moraes Monteiro |
| collection | DOAJ |
| description | Wild rodents serve as crucial reservoirs for zoonotic viruses. Anthropogenic and environmental disruptions, particularly those induced by mining activities, can destabilize rodent populations and facilitate the emergence of viral agents. In the Canaã dos Carajás and Curionópolis regions of Brazil, significant environmental changes have occurred due to mining expansion, potentially creating conditions conducive to the emergence of rodent-associated viral diseases. This study aimed to investigate the viral diversity in wild rodents captured in Canaã dos Carajás and Curionópolis, Pará, between 2017 and 2019. A total of 102 rodent samples were taxonomically identified through karyotyping and screened for anti-Orthohantavirus antibodies using the ELISA method. Subsequently, nucleotide sequencing and bioinformatics analyses were conducted on 14 selected samples to characterize the virome. This selection was based on the most commonly associated rodent genera as reservoirs of Orthohantavirus and Mammarenavirus. Of the 102 samples tested via ELISA, 100 were negative, and two showed optical density at the cutoff point. Sequencing of the 14 samples generated approximately 520 million reads, with 409 million retained after quality control. These reads were categorized into 53 viral families, including both DNA and RNA viruses, with Retroviridae, Baculoviridae, and Microviridae being the most abundant. Viral contigs were identified, including one fragment related to Arenaviridae and three to Filoviridae. Metagenomic analysis revealed high viral diversity in the sampled rodents, with the presence of viral families of public health concern, such as Arenaviridae and Filoviridae. The findings suggest that increased human activities associated with mining may contribute to the emergence of these viruses, underscoring the need for ongoing surveillance to prevent potential outbreaks. |
| format | Article |
| id | doaj-art-856606c5c17942969e202c36538bc7fe |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-856606c5c17942969e202c36538bc7fe2025-01-07T06:41:05ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011510.3389/fmicb.2024.15024621502462Viral diversity in wild rodents in the regions of Canaã de Carajás and Curionopólis, State of Pará, BrazilAdriana Freitas Moraes Monteiro0Adriana Freitas Moraes Monteiro1Fábio Silva da Silva2Ana Cecília Ribeiro Cruz3Ana Cecília Ribeiro Cruz4Sandro Patroca da Silva5Alice Louize Nunes Queiroz6Livia Medeiros Neves Casseb7Livia Medeiros Neves Casseb8Livia Carício Martins9Livia Carício Martins10Daniele Barbosa de Almeida Medeiros11Daniele Barbosa de Almeida Medeiros12Graduate Program in Virology, Evandro Chagas Institute — IEC/MS/SVSA, Ananindeua, BrazilEvandro Chagas Institute — IEC/MS/SVSA, Department of Arbovirology and Hemorragic Fevers, Ananindeua, BrazilEvandro Chagas Institute — IEC/MS/SVSA, Department of Arbovirology and Hemorragic Fevers, Ananindeua, BrazilGraduate Program in Virology, Evandro Chagas Institute — IEC/MS/SVSA, Ananindeua, BrazilEvandro Chagas Institute — IEC/MS/SVSA, Department of Arbovirology and Hemorragic Fevers, Ananindeua, BrazilEvandro Chagas Institute — IEC/MS/SVSA, Department of Arbovirology and Hemorragic Fevers, Ananindeua, BrazilEvandro Chagas Institute — IEC/MS/SVSA, Department of Arbovirology and Hemorragic Fevers, Ananindeua, BrazilGraduate Program in Virology, Evandro Chagas Institute — IEC/MS/SVSA, Ananindeua, BrazilEvandro Chagas Institute — IEC/MS/SVSA, Department of Arbovirology and Hemorragic Fevers, Ananindeua, BrazilGraduate Program in Virology, Evandro Chagas Institute — IEC/MS/SVSA, Ananindeua, BrazilEvandro Chagas Institute — IEC/MS/SVSA, Department of Arbovirology and Hemorragic Fevers, Ananindeua, BrazilGraduate Program in Virology, Evandro Chagas Institute — IEC/MS/SVSA, Ananindeua, BrazilEvandro Chagas Institute — IEC/MS/SVSA, Department of Arbovirology and Hemorragic Fevers, Ananindeua, BrazilWild rodents serve as crucial reservoirs for zoonotic viruses. Anthropogenic and environmental disruptions, particularly those induced by mining activities, can destabilize rodent populations and facilitate the emergence of viral agents. In the Canaã dos Carajás and Curionópolis regions of Brazil, significant environmental changes have occurred due to mining expansion, potentially creating conditions conducive to the emergence of rodent-associated viral diseases. This study aimed to investigate the viral diversity in wild rodents captured in Canaã dos Carajás and Curionópolis, Pará, between 2017 and 2019. A total of 102 rodent samples were taxonomically identified through karyotyping and screened for anti-Orthohantavirus antibodies using the ELISA method. Subsequently, nucleotide sequencing and bioinformatics analyses were conducted on 14 selected samples to characterize the virome. This selection was based on the most commonly associated rodent genera as reservoirs of Orthohantavirus and Mammarenavirus. Of the 102 samples tested via ELISA, 100 were negative, and two showed optical density at the cutoff point. Sequencing of the 14 samples generated approximately 520 million reads, with 409 million retained after quality control. These reads were categorized into 53 viral families, including both DNA and RNA viruses, with Retroviridae, Baculoviridae, and Microviridae being the most abundant. Viral contigs were identified, including one fragment related to Arenaviridae and three to Filoviridae. Metagenomic analysis revealed high viral diversity in the sampled rodents, with the presence of viral families of public health concern, such as Arenaviridae and Filoviridae. The findings suggest that increased human activities associated with mining may contribute to the emergence of these viruses, underscoring the need for ongoing surveillance to prevent potential outbreaks.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1502462/fullArenaviridaeepidemiologic vigilanceFiloviridaemetagenomicsrodent |
| spellingShingle | Adriana Freitas Moraes Monteiro Adriana Freitas Moraes Monteiro Fábio Silva da Silva Ana Cecília Ribeiro Cruz Ana Cecília Ribeiro Cruz Sandro Patroca da Silva Alice Louize Nunes Queiroz Livia Medeiros Neves Casseb Livia Medeiros Neves Casseb Livia Carício Martins Livia Carício Martins Daniele Barbosa de Almeida Medeiros Daniele Barbosa de Almeida Medeiros Viral diversity in wild rodents in the regions of Canaã de Carajás and Curionopólis, State of Pará, Brazil Frontiers in Microbiology Arenaviridae epidemiologic vigilance Filoviridae metagenomics rodent |
| title | Viral diversity in wild rodents in the regions of Canaã de Carajás and Curionopólis, State of Pará, Brazil |
| title_full | Viral diversity in wild rodents in the regions of Canaã de Carajás and Curionopólis, State of Pará, Brazil |
| title_fullStr | Viral diversity in wild rodents in the regions of Canaã de Carajás and Curionopólis, State of Pará, Brazil |
| title_full_unstemmed | Viral diversity in wild rodents in the regions of Canaã de Carajás and Curionopólis, State of Pará, Brazil |
| title_short | Viral diversity in wild rodents in the regions of Canaã de Carajás and Curionopólis, State of Pará, Brazil |
| title_sort | viral diversity in wild rodents in the regions of canaa de carajas and curionopolis state of para brazil |
| topic | Arenaviridae epidemiologic vigilance Filoviridae metagenomics rodent |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1502462/full |
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