The Paradox of Clean Eating: Neuroactive Dysbiosis and Pesticide Residues in Fruit- and Vegetable-Based Diets

(1) Background: Exposure to pesticide residues through food remains a critical issue in public health, especially given their potential cumulative neurotoxic effects. (2) Methods: This study investigated the presence of pesticide residues in commonly consumed vegetables, fruits, and cereals based on...

Full description

Saved in:
Bibliographic Details
Main Authors: Ramona Alina Tomuța, Andrada Florina Moldovan, Loredana Matiș, Lavinia Maris, Timea Claudia Ghitea, Florin Banica
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Toxics
Subjects:
Online Access:https://www.mdpi.com/2305-6304/13/6/504
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850165117673537536
author Ramona Alina Tomuța
Andrada Florina Moldovan
Loredana Matiș
Lavinia Maris
Timea Claudia Ghitea
Florin Banica
author_facet Ramona Alina Tomuța
Andrada Florina Moldovan
Loredana Matiș
Lavinia Maris
Timea Claudia Ghitea
Florin Banica
author_sort Ramona Alina Tomuța
collection DOAJ
description (1) Background: Exposure to pesticide residues through food remains a critical issue in public health, especially given their potential cumulative neurotoxic effects. (2) Methods: This study investigated the presence of pesticide residues in commonly consumed vegetables, fruits, and cereals based on official laboratory reports and evaluated the intestinal microbiome profiles of individuals whose diets consisted of over 50% plant-based foods. (3) Results: Analytical results from accredited laboratories in Romania demonstrated that all tested food samples were compliant with European regulations (Regulation (EC) 396/2005), with either undetectable or below-quantification-limit pesticide residues. However, organophosphates such as chlorpyrifos and diazinon were frequently tested, indicating persistent regulatory concern due to their known neurotoxic potential. A parallel analysis of stool samples revealed significant imbalances in neuroactive gut bacteria, including consistently low levels of <i>Bifidobacterium</i> and <i>Lactobacillus</i> species, and elevated levels of <i>Oscillibacter</i> and <i>Alistipes</i>, which are implicated in modulating GABA and serotonin pathways. Markers of proinflammatory activity, such as LPS-positive bacteria and histamine producers, were also elevated. (4) Conclusions: These findings suggest that even in diets rich in plant-based foods, microbial dysbiosis with neuroactive relevance can occur, potentially linked to environmental or dietary factors. The study underscores the need for a comprehensive evaluation of food safety and microbiome function as interconnected determinants of neurological health.
format Article
id doaj-art-84b1fde716e344d4a293fc32673541d9
institution OA Journals
issn 2305-6304
language English
publishDate 2025-06-01
publisher MDPI AG
record_format Article
series Toxics
spelling doaj-art-84b1fde716e344d4a293fc32673541d92025-08-20T02:21:49ZengMDPI AGToxics2305-63042025-06-0113650410.3390/toxics13060504The Paradox of Clean Eating: Neuroactive Dysbiosis and Pesticide Residues in Fruit- and Vegetable-Based DietsRamona Alina Tomuța0Andrada Florina Moldovan1Loredana Matiș2Lavinia Maris3Timea Claudia Ghitea4Florin Banica5Doctoral School of Biological and Biomedical Sciences, University of Oradea, 1 University Street, 410087 Oradea, RomaniaMedicine Department, Faculty of Medicine and Pharmacy, University of Oradea, 1 University Street, 410087 Oradea, RomaniaMedicine Department, Faculty of Medicine and Pharmacy, University of Oradea, 1 University Street, 410087 Oradea, RomaniaFaculty of Medicine, “Vasile Goldis” Western University of Arad, 94 Revolutiei Blvd., 310130 Arad, RomaniaPharmacy Department, Faculty of Medicine and Pharmacy, University of Oradea, 1 University Street, 410087 Oradea, RomaniaPharmacy Department, Faculty of Medicine and Pharmacy, University of Oradea, 1 University Street, 410087 Oradea, Romania(1) Background: Exposure to pesticide residues through food remains a critical issue in public health, especially given their potential cumulative neurotoxic effects. (2) Methods: This study investigated the presence of pesticide residues in commonly consumed vegetables, fruits, and cereals based on official laboratory reports and evaluated the intestinal microbiome profiles of individuals whose diets consisted of over 50% plant-based foods. (3) Results: Analytical results from accredited laboratories in Romania demonstrated that all tested food samples were compliant with European regulations (Regulation (EC) 396/2005), with either undetectable or below-quantification-limit pesticide residues. However, organophosphates such as chlorpyrifos and diazinon were frequently tested, indicating persistent regulatory concern due to their known neurotoxic potential. A parallel analysis of stool samples revealed significant imbalances in neuroactive gut bacteria, including consistently low levels of <i>Bifidobacterium</i> and <i>Lactobacillus</i> species, and elevated levels of <i>Oscillibacter</i> and <i>Alistipes</i>, which are implicated in modulating GABA and serotonin pathways. Markers of proinflammatory activity, such as LPS-positive bacteria and histamine producers, were also elevated. (4) Conclusions: These findings suggest that even in diets rich in plant-based foods, microbial dysbiosis with neuroactive relevance can occur, potentially linked to environmental or dietary factors. The study underscores the need for a comprehensive evaluation of food safety and microbiome function as interconnected determinants of neurological health.https://www.mdpi.com/2305-6304/13/6/504pesticide residuesorganophosphatesgut microbiomeneuroactive bacteriachlorpyrifosdietary exposure
spellingShingle Ramona Alina Tomuța
Andrada Florina Moldovan
Loredana Matiș
Lavinia Maris
Timea Claudia Ghitea
Florin Banica
The Paradox of Clean Eating: Neuroactive Dysbiosis and Pesticide Residues in Fruit- and Vegetable-Based Diets
Toxics
pesticide residues
organophosphates
gut microbiome
neuroactive bacteria
chlorpyrifos
dietary exposure
title The Paradox of Clean Eating: Neuroactive Dysbiosis and Pesticide Residues in Fruit- and Vegetable-Based Diets
title_full The Paradox of Clean Eating: Neuroactive Dysbiosis and Pesticide Residues in Fruit- and Vegetable-Based Diets
title_fullStr The Paradox of Clean Eating: Neuroactive Dysbiosis and Pesticide Residues in Fruit- and Vegetable-Based Diets
title_full_unstemmed The Paradox of Clean Eating: Neuroactive Dysbiosis and Pesticide Residues in Fruit- and Vegetable-Based Diets
title_short The Paradox of Clean Eating: Neuroactive Dysbiosis and Pesticide Residues in Fruit- and Vegetable-Based Diets
title_sort paradox of clean eating neuroactive dysbiosis and pesticide residues in fruit and vegetable based diets
topic pesticide residues
organophosphates
gut microbiome
neuroactive bacteria
chlorpyrifos
dietary exposure
url https://www.mdpi.com/2305-6304/13/6/504
work_keys_str_mv AT ramonaalinatomuta theparadoxofcleaneatingneuroactivedysbiosisandpesticideresiduesinfruitandvegetablebaseddiets
AT andradaflorinamoldovan theparadoxofcleaneatingneuroactivedysbiosisandpesticideresiduesinfruitandvegetablebaseddiets
AT loredanamatis theparadoxofcleaneatingneuroactivedysbiosisandpesticideresiduesinfruitandvegetablebaseddiets
AT laviniamaris theparadoxofcleaneatingneuroactivedysbiosisandpesticideresiduesinfruitandvegetablebaseddiets
AT timeaclaudiaghitea theparadoxofcleaneatingneuroactivedysbiosisandpesticideresiduesinfruitandvegetablebaseddiets
AT florinbanica theparadoxofcleaneatingneuroactivedysbiosisandpesticideresiduesinfruitandvegetablebaseddiets
AT ramonaalinatomuta paradoxofcleaneatingneuroactivedysbiosisandpesticideresiduesinfruitandvegetablebaseddiets
AT andradaflorinamoldovan paradoxofcleaneatingneuroactivedysbiosisandpesticideresiduesinfruitandvegetablebaseddiets
AT loredanamatis paradoxofcleaneatingneuroactivedysbiosisandpesticideresiduesinfruitandvegetablebaseddiets
AT laviniamaris paradoxofcleaneatingneuroactivedysbiosisandpesticideresiduesinfruitandvegetablebaseddiets
AT timeaclaudiaghitea paradoxofcleaneatingneuroactivedysbiosisandpesticideresiduesinfruitandvegetablebaseddiets
AT florinbanica paradoxofcleaneatingneuroactivedysbiosisandpesticideresiduesinfruitandvegetablebaseddiets