Protocol for generating brain metastatic tumor cells through repeated intracardiac injections in mice
Summary: Here, we present a protocol for generating the lung cancer cell line, LLC1-BMT5, with highly brain metastatic tropism through multiple rounds of in vivo selection. We describe steps for establishing the brain metastases (BrMs) mouse model through intracardiac injection of cancer cells. We t...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724006968 |
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