Cell-based ELISpot protocol to detect and quantify antigen-specific antibody-secreting cells in murine whole-organ single-cell suspensions
Summary: Antibody-secreting cells (ASCs) are the hallmark of the adaptive immune response to vaccines or infections. Here, we present a protocol to quantify the number of ASCs using an enzyme-linked immunospot assay (ELISpot). We describe the preparation of cell samples, ELISpot plates, and their qu...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-09-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725002035 |
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