Peripheral Classic and Intermediate Monocyte Subsets as Immune Biomarkers of Systemic Lupus Erythematosus Disease Activity
Background: Monocytes are evolutionarily preserved innate immune cells that play essential roles in immune response regulation. Three activated monocyte subsets—classical (CD14++CD16–), intermediate (CD14++CD16+), and nonclassical (CD14+CD16++)—are associated with systemic lupus erythematosus (SLE)...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Interna Publishing
2024-10-01
|
| Series: | Acta Medica Indonesiana |
| Subjects: | |
| Online Access: | https://actamedindones.org/index.php/ijim/article/view/2707 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832585137865359360 |
|---|---|
| author | Amaylia Oehadian Mohammad Ghozali Sutiadi Kusuma Lusi Mersiana Nadia Gita Ghassani Fransisca Fransisca Yitzchak Millenard Sigilipu Andini Kartikasari Laniyati Hamijoyo |
| author_facet | Amaylia Oehadian Mohammad Ghozali Sutiadi Kusuma Lusi Mersiana Nadia Gita Ghassani Fransisca Fransisca Yitzchak Millenard Sigilipu Andini Kartikasari Laniyati Hamijoyo |
| author_sort | Amaylia Oehadian |
| collection | DOAJ |
| description | Background: Monocytes are evolutionarily preserved innate immune cells that play essential roles in immune response regulation. Three activated monocyte subsets—classical (CD14++CD16–), intermediate (CD14++CD16+), and nonclassical (CD14+CD16++)—are associated with systemic lupus erythematosus (SLE) progression. This study aims to determine the association of monocyte subsets with SLE disease activity. Methods: A cross-sectional study involving 25 patients with SLE was conducted. Blood samples were collected, and monocyte subsets were identified using flow cytometry. Patients were grouped by disease activity using the Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2K) into inactive (SLEDAI-2K ≤ 4) and active (SLEDAI-2K > 4). The cutoff for monocyte subsets was determined using Receiver Operating Characteristic (ROC) analysis. Results: Nine active and 16 inactive subjects were identified. Compared with individuals without active disease, individuals with active disease had significantly lower mean classical monocyte subsets (71.9% vs 88%, p = 0.008), and higher median intermediate monocytes (29.1% vs 11.1%, p = 0.019). The median nonclassical monocyte subsets were not significantly different between the two groups. The cutoff for classical monocytes in active disease was ≤72.2%, AUC = 0.788, p = 0.021, with 66.7% sensitivity and 87.5% specificity; for intermediate monocytes, it was >22.3%, AUC = 0.788, p = 0.014, with 66.7% sensitivity and 100% specificity. Conclusion: Classical and intermediate monocytes could be considered as immune cellular markers for identifying active SLE. |
| format | Article |
| id | doaj-art-83c8a1bc1050483f85d4b0ca1d0ff550 |
| institution | Kabale University |
| issn | 0125-9326 2338-2732 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Interna Publishing |
| record_format | Article |
| series | Acta Medica Indonesiana |
| spelling | doaj-art-83c8a1bc1050483f85d4b0ca1d0ff5502025-01-27T04:12:06ZengInterna PublishingActa Medica Indonesiana0125-93262338-27322024-10-01564694Peripheral Classic and Intermediate Monocyte Subsets as Immune Biomarkers of Systemic Lupus Erythematosus Disease ActivityAmaylia Oehadian0Mohammad Ghozali1Sutiadi Kusuma2Lusi Mersiana3Nadia Gita Ghassani4Fransisca Fransisca5Yitzchak Millenard Sigilipu6Andini Kartikasari7Laniyati Hamijoyo8Hematology Division, Department of Internal Medicine, Faculty of Medicine, Padjadjaran University - Hasan Sadikin Hospital, Bandung, IndonesiaDepartment of Biomedical Sciences, Faculty of Medicine, Padjadjaran University, Bandung, IndonesiaHematology Division, Department of Internal Medicine, Faculty of Medicine, Padjadjaran University - Hasan Sadikin Hospital, Bandung, Indonesia.Hematology Division, Department of Internal Medicine, Faculty of Medicine, Padjadjaran University - Hasan Sadikin Hospital, Bandung, Indonesia.Lupus Study Group, Immunology Study Center, Faculty of Medicine, Padjadjaran University, Bandung, IndonesiaLupus Study Group, Immunology Study Center, Faculty of Medicine, Padjadjaran University, Bandung, IndonesiaFaculty of Medicine, Padjadjaran University, Bandung, IndonesiaBandung City General Regional Hospital, Bandung, Indonesia1. Lupus Study Group, Immunology Study Center, Faculty of Medicine, Padjadjaran University, Bandung, Indonesia. 2. Rheumatology Division, Department of Internal Medicine, Faculty of Medicine, Padjadjaran University - Hasan Sadikin Hospital, Bandung, Indonesia.Background: Monocytes are evolutionarily preserved innate immune cells that play essential roles in immune response regulation. Three activated monocyte subsets—classical (CD14++CD16–), intermediate (CD14++CD16+), and nonclassical (CD14+CD16++)—are associated with systemic lupus erythematosus (SLE) progression. This study aims to determine the association of monocyte subsets with SLE disease activity. Methods: A cross-sectional study involving 25 patients with SLE was conducted. Blood samples were collected, and monocyte subsets were identified using flow cytometry. Patients were grouped by disease activity using the Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2K) into inactive (SLEDAI-2K ≤ 4) and active (SLEDAI-2K > 4). The cutoff for monocyte subsets was determined using Receiver Operating Characteristic (ROC) analysis. Results: Nine active and 16 inactive subjects were identified. Compared with individuals without active disease, individuals with active disease had significantly lower mean classical monocyte subsets (71.9% vs 88%, p = 0.008), and higher median intermediate monocytes (29.1% vs 11.1%, p = 0.019). The median nonclassical monocyte subsets were not significantly different between the two groups. The cutoff for classical monocytes in active disease was ≤72.2%, AUC = 0.788, p = 0.021, with 66.7% sensitivity and 87.5% specificity; for intermediate monocytes, it was >22.3%, AUC = 0.788, p = 0.014, with 66.7% sensitivity and 100% specificity. Conclusion: Classical and intermediate monocytes could be considered as immune cellular markers for identifying active SLE.https://actamedindones.org/index.php/ijim/article/view/2707monocytes subsetsleclassical monocytesintermediate monocytesdisease activity |
| spellingShingle | Amaylia Oehadian Mohammad Ghozali Sutiadi Kusuma Lusi Mersiana Nadia Gita Ghassani Fransisca Fransisca Yitzchak Millenard Sigilipu Andini Kartikasari Laniyati Hamijoyo Peripheral Classic and Intermediate Monocyte Subsets as Immune Biomarkers of Systemic Lupus Erythematosus Disease Activity Acta Medica Indonesiana monocytes subset sle classical monocytes intermediate monocytes disease activity |
| title | Peripheral Classic and Intermediate Monocyte Subsets as Immune Biomarkers of Systemic Lupus Erythematosus Disease Activity |
| title_full | Peripheral Classic and Intermediate Monocyte Subsets as Immune Biomarkers of Systemic Lupus Erythematosus Disease Activity |
| title_fullStr | Peripheral Classic and Intermediate Monocyte Subsets as Immune Biomarkers of Systemic Lupus Erythematosus Disease Activity |
| title_full_unstemmed | Peripheral Classic and Intermediate Monocyte Subsets as Immune Biomarkers of Systemic Lupus Erythematosus Disease Activity |
| title_short | Peripheral Classic and Intermediate Monocyte Subsets as Immune Biomarkers of Systemic Lupus Erythematosus Disease Activity |
| title_sort | peripheral classic and intermediate monocyte subsets as immune biomarkers of systemic lupus erythematosus disease activity |
| topic | monocytes subset sle classical monocytes intermediate monocytes disease activity |
| url | https://actamedindones.org/index.php/ijim/article/view/2707 |
| work_keys_str_mv | AT amayliaoehadian peripheralclassicandintermediatemonocytesubsetsasimmunebiomarkersofsystemiclupuserythematosusdiseaseactivity AT mohammadghozali peripheralclassicandintermediatemonocytesubsetsasimmunebiomarkersofsystemiclupuserythematosusdiseaseactivity AT sutiadikusuma peripheralclassicandintermediatemonocytesubsetsasimmunebiomarkersofsystemiclupuserythematosusdiseaseactivity AT lusimersiana peripheralclassicandintermediatemonocytesubsetsasimmunebiomarkersofsystemiclupuserythematosusdiseaseactivity AT nadiagitaghassani peripheralclassicandintermediatemonocytesubsetsasimmunebiomarkersofsystemiclupuserythematosusdiseaseactivity AT fransiscafransisca peripheralclassicandintermediatemonocytesubsetsasimmunebiomarkersofsystemiclupuserythematosusdiseaseactivity AT yitzchakmillenardsigilipu peripheralclassicandintermediatemonocytesubsetsasimmunebiomarkersofsystemiclupuserythematosusdiseaseactivity AT andinikartikasari peripheralclassicandintermediatemonocytesubsetsasimmunebiomarkersofsystemiclupuserythematosusdiseaseactivity AT laniyatihamijoyo peripheralclassicandintermediatemonocytesubsetsasimmunebiomarkersofsystemiclupuserythematosusdiseaseactivity |