Purification and Characterization of Endoglucanase from local isolate of Aspergillus flavus
Endoglucanase produced from Aspergillus flavus was purified by several steps including precipitation with 25 % ammonium sulphate followed by Ion –exchange chromatography, the obtained specific activity was 377.35 U/ mg protein, with a yield of 51.32 % .This step was followed by gel filtration chroma...
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| Format: | Article |
| Language: | English |
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University of Baghdad, College of Science for Women
2013-09-01
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| Series: | مجلة بغداد للعلوم |
| Online Access: | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2594 |
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| author | Baghdad Science Journal |
| author_facet | Baghdad Science Journal |
| author_sort | Baghdad Science Journal |
| collection | DOAJ |
| description | Endoglucanase produced from Aspergillus flavus was purified by several steps including precipitation with 25 % ammonium sulphate followed by Ion –exchange chromatography, the obtained specific activity was 377.35 U/ mg protein, with a yield of 51.32 % .This step was followed by gel filtration chromatography (Sepharose -6B), when a value of specific activity was 400 U/ mg protein, with a yield of 48 %. Certain properties of this purified enzyme were investigated, the optimum pH of activity was 7 and the pH of its stability was 4.5, while the temperature stability was 40 °C for 60 min. The enzyme retained 100% of its original activity after incubation at 40 °C for 60 min; the optimum temperature for enzyme activity was 40 °C. |
| format | Article |
| id | doaj-art-82e41efa693344a2921a16bb965dff5c |
| institution | Kabale University |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2013-09-01 |
| publisher | University of Baghdad, College of Science for Women |
| record_format | Article |
| series | مجلة بغداد للعلوم |
| spelling | doaj-art-82e41efa693344a2921a16bb965dff5c2025-08-20T03:57:03ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862013-09-0110310.21123/bsj.10.3.844-853Purification and Characterization of Endoglucanase from local isolate of Aspergillus flavusBaghdad Science JournalEndoglucanase produced from Aspergillus flavus was purified by several steps including precipitation with 25 % ammonium sulphate followed by Ion –exchange chromatography, the obtained specific activity was 377.35 U/ mg protein, with a yield of 51.32 % .This step was followed by gel filtration chromatography (Sepharose -6B), when a value of specific activity was 400 U/ mg protein, with a yield of 48 %. Certain properties of this purified enzyme were investigated, the optimum pH of activity was 7 and the pH of its stability was 4.5, while the temperature stability was 40 °C for 60 min. The enzyme retained 100% of its original activity after incubation at 40 °C for 60 min; the optimum temperature for enzyme activity was 40 °C.http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2594 |
| spellingShingle | Baghdad Science Journal Purification and Characterization of Endoglucanase from local isolate of Aspergillus flavus مجلة بغداد للعلوم |
| title | Purification and Characterization of Endoglucanase from local isolate of Aspergillus flavus |
| title_full | Purification and Characterization of Endoglucanase from local isolate of Aspergillus flavus |
| title_fullStr | Purification and Characterization of Endoglucanase from local isolate of Aspergillus flavus |
| title_full_unstemmed | Purification and Characterization of Endoglucanase from local isolate of Aspergillus flavus |
| title_short | Purification and Characterization of Endoglucanase from local isolate of Aspergillus flavus |
| title_sort | purification and characterization of endoglucanase from local isolate of aspergillus flavus |
| url | http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2594 |
| work_keys_str_mv | AT baghdadsciencejournal purificationandcharacterizationofendoglucanasefromlocalisolateofaspergillusflavus |