Medicarpin suppresses lung cancer cell growth in vitro and in vivo by inducing cell apoptosis
Lung cancer (LC) is the leading cause of cancer deaths worldwide. Surgery, chemoradiotherapy, targeted therapy, and immunotherapy are considered dominant treatment strategies for LC in the clinic. However, drug resistance and meta-stasis are two major challenges in cancer therapies. Medicarpin (MED)...
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| Format: | Article |
| Language: | English |
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Sciendo
2024-03-01
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| Series: | Acta Pharmaceutica |
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| Online Access: | https://doi.org/10.2478/acph-2024-0006 |
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| author | Shen Zongyi Yin Liqi Chang Manxia Wang Haifeng Hao Mingxuan Liang Youfeng Guo Rui Bi Ying Wang Jiansong Yu Changyuan Li Jinmei Zhai Qiongli Cheng Runfen Zhang Jinku Sun Jirui Yang Zhao |
| author_facet | Shen Zongyi Yin Liqi Chang Manxia Wang Haifeng Hao Mingxuan Liang Youfeng Guo Rui Bi Ying Wang Jiansong Yu Changyuan Li Jinmei Zhai Qiongli Cheng Runfen Zhang Jinku Sun Jirui Yang Zhao |
| author_sort | Shen Zongyi |
| collection | DOAJ |
| description | Lung cancer (LC) is the leading cause of cancer deaths worldwide. Surgery, chemoradiotherapy, targeted therapy, and immunotherapy are considered dominant treatment strategies for LC in the clinic. However, drug resistance and meta-stasis are two major challenges in cancer therapies. Medicarpin (MED) is an isoflavone compound isolated from alfalfa, which is usually used in traditional medicine. This study was de sig ned to evaluate the anti-LC effect and reveal the underlying mechanisms of MED in vivo and in vitro. We found that MED could significantly inhibit proliferation, induce apoptosis, and cell cycle arrest of A549 and H157 cell lines. Basically, MED induced cell apoptosis of LC cells by upregu lating the expression of pro-apoptotic proteins BAX and Bak1, leading to the cleavage of caspase-3 (Casp3). Moreover, MED inhibited the proliferation of LC cells via downregulating the expression of proliferative protein Bid. Overall, MED inhibited LC cell growth in vitro and in vivo via suppressing cell proliferation and inducing cell apoptosis, suggesting the therapeutic potential of MED in treating LC. |
| format | Article |
| id | doaj-art-82305041bce84fcc8ebe66a2405a81d0 |
| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj-art-82305041bce84fcc8ebe66a2405a81d02025-02-02T21:44:37ZengSciendoActa Pharmaceutica1846-95582024-03-0174114916410.2478/acph-2024-0006Medicarpin suppresses lung cancer cell growth in vitro and in vivo by inducing cell apoptosisShen Zongyi0Yin Liqi1Chang Manxia2Wang Haifeng3Hao Mingxuan4Liang Youfeng5Guo Rui6Bi Ying7Wang Jiansong8Yu Changyuan9Li Jinmei10Zhai Qiongli11Cheng Runfen12Zhang Jinku13Sun Jirui14Yang Zhao15College of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing 100029, ChinaDepartment of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, ChinaCollege of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing 100029, ChinaCollege of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing 100029, ChinaDepartment of Urology, The Second Affiliated Hospital of Kunming Medical University, Kunming 650101, ChinaCollege of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing 100029, ChinaDepartment of Pathology, Baoding No. 1 Central Hospital, Baoding 071000, Hebei, ChinaDepartment of Pathology, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, ChinaDepartment of Pathology, National Clinical Research Center of Cancer, Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, ChinaDepartment of Pathology, Baoding No. 1 Central Hospital, Baoding 071000, Hebei, ChinaDepartment of Pathology, Baoding No. 1 Central Hospital, Baoding 071000, Hebei, ChinaCollege of Life Science and Technology, Innovation Center of Molecular Diagnostics, Beijing University of Chemical Technology, Beijing 100029, ChinaLung cancer (LC) is the leading cause of cancer deaths worldwide. Surgery, chemoradiotherapy, targeted therapy, and immunotherapy are considered dominant treatment strategies for LC in the clinic. However, drug resistance and meta-stasis are two major challenges in cancer therapies. Medicarpin (MED) is an isoflavone compound isolated from alfalfa, which is usually used in traditional medicine. This study was de sig ned to evaluate the anti-LC effect and reveal the underlying mechanisms of MED in vivo and in vitro. We found that MED could significantly inhibit proliferation, induce apoptosis, and cell cycle arrest of A549 and H157 cell lines. Basically, MED induced cell apoptosis of LC cells by upregu lating the expression of pro-apoptotic proteins BAX and Bak1, leading to the cleavage of caspase-3 (Casp3). Moreover, MED inhibited the proliferation of LC cells via downregulating the expression of proliferative protein Bid. Overall, MED inhibited LC cell growth in vitro and in vivo via suppressing cell proliferation and inducing cell apoptosis, suggesting the therapeutic potential of MED in treating LC.https://doi.org/10.2478/acph-2024-0006lung cancermedicarpincell apoptosiscell cycle arrest |
| spellingShingle | Shen Zongyi Yin Liqi Chang Manxia Wang Haifeng Hao Mingxuan Liang Youfeng Guo Rui Bi Ying Wang Jiansong Yu Changyuan Li Jinmei Zhai Qiongli Cheng Runfen Zhang Jinku Sun Jirui Yang Zhao Medicarpin suppresses lung cancer cell growth in vitro and in vivo by inducing cell apoptosis Acta Pharmaceutica lung cancer medicarpin cell apoptosis cell cycle arrest |
| title | Medicarpin suppresses lung cancer cell growth in vitro and in vivo by inducing cell apoptosis |
| title_full | Medicarpin suppresses lung cancer cell growth in vitro and in vivo by inducing cell apoptosis |
| title_fullStr | Medicarpin suppresses lung cancer cell growth in vitro and in vivo by inducing cell apoptosis |
| title_full_unstemmed | Medicarpin suppresses lung cancer cell growth in vitro and in vivo by inducing cell apoptosis |
| title_short | Medicarpin suppresses lung cancer cell growth in vitro and in vivo by inducing cell apoptosis |
| title_sort | medicarpin suppresses lung cancer cell growth in vitro and in vivo by inducing cell apoptosis |
| topic | lung cancer medicarpin cell apoptosis cell cycle arrest |
| url | https://doi.org/10.2478/acph-2024-0006 |
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