Effect of Pooling and Freeze-Thaw Processes on the PRRSV RNA Detection in TOSc Samples

Effect of Pooling and Freeze-Thaw Processes on the PRRSV RNA Detection in TOSc Samples

Tonsil oral scrubbing (TOSc) is a simple and practical method recently developed for detecting PRRSV RNA in sows. This study evaluated how pooling and freeze-thaw procedures affect PRRSV RT-rtPCR results in TOSc samples. To assess pooling effects, 22 PRRSV-positive samples were divided into three Ct...

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Bibliographic Details
Main Authors: Peng Li, Onyekachukwu H. Osemeke, Thinh Tran Pham Tien, Ching-Yang Wang, Angie Supple, Marcelo N. Almeida, Daniel C. L. Linhares
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Veterinary Sciences
Subjects:
PRRSV detection
pooling
freeze-thaw
TOSc
Online Access:https://www.mdpi.com/2306-7381/12/7/607
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