The culturable microbiological landscape of guarana-based products and wastes: insights from the Amazon state (Brazil) production chain

Guarana, a fruit native to South America, is a key ingredient in various products, including beverages and supplements, due to the high caffeine content, which gives the drinks stimulating properties. While the chemical properties of guarana seeds are well-studied, their microbiological profile rema...

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Main Authors: Dionisio Pedro Amorim-Neto, Clara Mariana Gonçalves Lima, Simone de Nazaré Melo Ramos, Raimunda Susielen Ribeiro Araújo, Ana Luisa Perini Leme Giordano, Angélica Zaninelli Schreiber, Anderson S. Sant’Ana, Priscilla Efraim
Format: Article
Language:English
Published: Elsevier 2025-12-01
Series:Applied Food Research
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Online Access:http://www.sciencedirect.com/science/article/pii/S2772502225004676
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author Dionisio Pedro Amorim-Neto
Clara Mariana Gonçalves Lima
Simone de Nazaré Melo Ramos
Raimunda Susielen Ribeiro Araújo
Ana Luisa Perini Leme Giordano
Angélica Zaninelli Schreiber
Anderson S. Sant’Ana
Priscilla Efraim
author_facet Dionisio Pedro Amorim-Neto
Clara Mariana Gonçalves Lima
Simone de Nazaré Melo Ramos
Raimunda Susielen Ribeiro Araújo
Ana Luisa Perini Leme Giordano
Angélica Zaninelli Schreiber
Anderson S. Sant’Ana
Priscilla Efraim
author_sort Dionisio Pedro Amorim-Neto
collection DOAJ
description Guarana, a fruit native to South America, is a key ingredient in various products, including beverages and supplements, due to the high caffeine content, which gives the drinks stimulating properties. While the chemical properties of guarana seeds are well-studied, their microbiological profile remains largely unknown. For this reason, this study investigates the microbiological characteristics of guarana-based products and by-products, focusing on quantifying viable spoilage microorganisms, pathogens, and opportunists throughout the guarana production chain. The study also assessed water activity and pH across different guarana products, noting that most products had low water activity (0.501–0.918) and acidic pH levels (4.97–6.65), similar to other processed fruits such as coffee and cocoa. Despite these conditions, microbial populations, including spore-forming bacteria such as Bacillus cereus and potential pathogens like Cronobacter sp. and Enterobacter cloacae, were detected. These microorganisms pose significant food safety concerns, as some can survive harsh processing conditions and cause severe health issues. However, pathogens like Salmonella and Listeria monocytogenes were not detected. Notably, guarana powder, often consumed directly, exhibited high microbial counts, highlighting the need for improved hygiene and good manufacturing practices throughout the guarana production chain to ensure consumer safety.
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spelling doaj-art-81bc3ec9d4ed49a98de6e560d9c350e72025-08-20T03:12:31ZengElsevierApplied Food Research2772-50222025-12-015210116210.1016/j.afres.2025.101162The culturable microbiological landscape of guarana-based products and wastes: insights from the Amazon state (Brazil) production chainDionisio Pedro Amorim-Neto0Clara Mariana Gonçalves Lima1Simone de Nazaré Melo Ramos2Raimunda Susielen Ribeiro Araújo3Ana Luisa Perini Leme Giordano4Angélica Zaninelli Schreiber5Anderson S. Sant’Ana6Priscilla Efraim7Department of Food Science and Nutrition, Faculty of Food Engineering, Universidade Estadual de Campinas, Campinas, SP 13083-862, BrazilDepartment of Food Science and Nutrition, Faculty of Food Engineering, Universidade Estadual de Campinas, Campinas, SP 13083-862, BrazilDepartment of Food Engineering and Technology, Faculty of Food Engineering, Universidade Estadual de Campinas, Campinas, SP 13083-862, BrazilFaculty of Food Technology, Universidade Estadual do Amazonas, Faculdade de Tecnologia de Alimentos, Maués, AM 69190-000, BrazilDepartment of Pathology, Faculty of Medical Sciences, University of Campinas, Campinas, SP 13083-862, BrazilDepartment of Pathology, Faculty of Medical Sciences, University of Campinas, Campinas, SP 13083-862, BrazilDepartment of Food Science and Nutrition, Faculty of Food Engineering, Universidade Estadual de Campinas, Campinas, SP 13083-862, BrazilDepartment of Food Engineering and Technology, Faculty of Food Engineering, Universidade Estadual de Campinas, Campinas, SP 13083-862, Brazil; Corresponding author at: Laboratory of Fruits and Vegetables, Department of Food Engineering and Technology, Faculty of Food Engineering, Universidade Estadual de Campinas. Monteiro Lobato, 80, Zip Code: 13083-862, Cidade Universitária, Campinas, São Paulo, Brazil.Guarana, a fruit native to South America, is a key ingredient in various products, including beverages and supplements, due to the high caffeine content, which gives the drinks stimulating properties. While the chemical properties of guarana seeds are well-studied, their microbiological profile remains largely unknown. For this reason, this study investigates the microbiological characteristics of guarana-based products and by-products, focusing on quantifying viable spoilage microorganisms, pathogens, and opportunists throughout the guarana production chain. The study also assessed water activity and pH across different guarana products, noting that most products had low water activity (0.501–0.918) and acidic pH levels (4.97–6.65), similar to other processed fruits such as coffee and cocoa. Despite these conditions, microbial populations, including spore-forming bacteria such as Bacillus cereus and potential pathogens like Cronobacter sp. and Enterobacter cloacae, were detected. These microorganisms pose significant food safety concerns, as some can survive harsh processing conditions and cause severe health issues. However, pathogens like Salmonella and Listeria monocytogenes were not detected. Notably, guarana powder, often consumed directly, exhibited high microbial counts, highlighting the need for improved hygiene and good manufacturing practices throughout the guarana production chain to ensure consumer safety.http://www.sciencedirect.com/science/article/pii/S2772502225004676ContaminationLow-moisture foodsTraditional foodSporeforming bacteriaMaldi-tof
spellingShingle Dionisio Pedro Amorim-Neto
Clara Mariana Gonçalves Lima
Simone de Nazaré Melo Ramos
Raimunda Susielen Ribeiro Araújo
Ana Luisa Perini Leme Giordano
Angélica Zaninelli Schreiber
Anderson S. Sant’Ana
Priscilla Efraim
The culturable microbiological landscape of guarana-based products and wastes: insights from the Amazon state (Brazil) production chain
Applied Food Research
Contamination
Low-moisture foods
Traditional food
Sporeforming bacteria
Maldi-tof
title The culturable microbiological landscape of guarana-based products and wastes: insights from the Amazon state (Brazil) production chain
title_full The culturable microbiological landscape of guarana-based products and wastes: insights from the Amazon state (Brazil) production chain
title_fullStr The culturable microbiological landscape of guarana-based products and wastes: insights from the Amazon state (Brazil) production chain
title_full_unstemmed The culturable microbiological landscape of guarana-based products and wastes: insights from the Amazon state (Brazil) production chain
title_short The culturable microbiological landscape of guarana-based products and wastes: insights from the Amazon state (Brazil) production chain
title_sort culturable microbiological landscape of guarana based products and wastes insights from the amazon state brazil production chain
topic Contamination
Low-moisture foods
Traditional food
Sporeforming bacteria
Maldi-tof
url http://www.sciencedirect.com/science/article/pii/S2772502225004676
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