Effect of platelet-activating factor on the growth of human erythroid and myeloid CD34+ progenitors
We have assessed the effect of platelet-activating factor (PAF), a biologically active phospholipid present in the human marrow, on the growth of human marrow and blood CD34+ progenitors. While the metabolization rate of PAF by CD34+ cells is low (weak acetylhydrolase and acylation processes) it is...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
1998-01-01
|
| Series: | Mediators of Inflammation |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09629359891243 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832550635458789376 |
|---|---|
| author | F. Dupuis N. Gachard A. Allegraud C. Dulery V. Praloran Y. Denizot |
| author_facet | F. Dupuis N. Gachard A. Allegraud C. Dulery V. Praloran Y. Denizot |
| author_sort | F. Dupuis |
| collection | DOAJ |
| description | We have assessed the effect of platelet-activating factor (PAF), a biologically active phospholipid present in the human marrow, on the growth of human marrow and blood CD34+ progenitors. While the metabolization rate of PAF by CD34+ cells is low (weak acetylhydrolase and acylation processes) it is readily catabolized by the acetylhydrolase activity present in the growth medium (10% fetal calf serum + 10% 5637-conditioned medium). Treatment of marrow CD34+ cells with the non-metabolizable PAF agonist C-PAF (1 nM to 100 nM) immediately before semi-solid culture significantly (p<0.01) decreased the number of BFU-E but not of CFU-GM colonies. Treatment of marrow or blood CD34+ cells with C-PAF (10-100 nM) for 3 days in liquid medium before semi-solid culture significantly (p<0.01) decreased the number of BFUE and CFU-GM colonies. Treatment of blood CD34+ cells with the two PAF receptor antagonists CV 3988 and BN 52021 (1 μ M) had no significant effect on the number of BFU-E and CFU-GM colonies suggesting no role of endogenous PAF in these processes. These results show that exogenous PAF downregulates human erythropoiesis and myelopoiesis, a result that might be of importance during inflammatory states. |
| format | Article |
| id | doaj-art-816826f9ac6e48de8c2e6bc8bd6e004b |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-816826f9ac6e48de8c2e6bc8bd6e004b2025-02-03T06:06:11ZengWileyMediators of Inflammation0962-93511466-18611998-01-01729910310.1080/09629359891243Effect of platelet-activating factor on the growth of human erythroid and myeloid CD34+ progenitorsF. Dupuis0N. Gachard1A. Allegraud2C. Dulery3V. Praloran4Y. Denizot5Laboratoire d'Hématologie Expérimentale, Faculté de Médecine, 2 rue Dr Marcland, Limoges 87025, FranceLaboratoire d'Hématologie Expérimentale, Faculté de Médecine, 2 rue Dr Marcland, Limoges 87025, FranceLaboratoire d'Hématologie Expérimentale, Faculté de Médecine, 2 rue Dr Marcland, Limoges 87025, FranceLaboratoire d'Hématologie Expérimentale, Faculté de Médecine, 2 rue Dr Marcland, Limoges 87025, FranceLaboratoire d'Hématologie Expérimentale, Faculté de Médecine, 2 rue Dr Marcland, Limoges 87025, FranceLaboratoire d'Hématologie Expérimentale, Faculté de Médecine, 2 rue Dr Marcland, Limoges 87025, FranceWe have assessed the effect of platelet-activating factor (PAF), a biologically active phospholipid present in the human marrow, on the growth of human marrow and blood CD34+ progenitors. While the metabolization rate of PAF by CD34+ cells is low (weak acetylhydrolase and acylation processes) it is readily catabolized by the acetylhydrolase activity present in the growth medium (10% fetal calf serum + 10% 5637-conditioned medium). Treatment of marrow CD34+ cells with the non-metabolizable PAF agonist C-PAF (1 nM to 100 nM) immediately before semi-solid culture significantly (p<0.01) decreased the number of BFU-E but not of CFU-GM colonies. Treatment of marrow or blood CD34+ cells with C-PAF (10-100 nM) for 3 days in liquid medium before semi-solid culture significantly (p<0.01) decreased the number of BFUE and CFU-GM colonies. Treatment of blood CD34+ cells with the two PAF receptor antagonists CV 3988 and BN 52021 (1 μ M) had no significant effect on the number of BFU-E and CFU-GM colonies suggesting no role of endogenous PAF in these processes. These results show that exogenous PAF downregulates human erythropoiesis and myelopoiesis, a result that might be of importance during inflammatory states.http://dx.doi.org/10.1080/09629359891243PAFCD34+Myeloid progenitorErythroid progenitor. |
| spellingShingle | F. Dupuis N. Gachard A. Allegraud C. Dulery V. Praloran Y. Denizot Effect of platelet-activating factor on the growth of human erythroid and myeloid CD34+ progenitors Mediators of Inflammation PAF CD34+ Myeloid progenitor Erythroid progenitor. |
| title | Effect of platelet-activating factor on the growth of human erythroid and
myeloid CD34+ progenitors |
| title_full | Effect of platelet-activating factor on the growth of human erythroid and
myeloid CD34+ progenitors |
| title_fullStr | Effect of platelet-activating factor on the growth of human erythroid and
myeloid CD34+ progenitors |
| title_full_unstemmed | Effect of platelet-activating factor on the growth of human erythroid and
myeloid CD34+ progenitors |
| title_short | Effect of platelet-activating factor on the growth of human erythroid and
myeloid CD34+ progenitors |
| title_sort | effect of platelet activating factor on the growth of human erythroid and myeloid cd34 progenitors |
| topic | PAF CD34+ Myeloid progenitor Erythroid progenitor. |
| url | http://dx.doi.org/10.1080/09629359891243 |
| work_keys_str_mv | AT fdupuis effectofplateletactivatingfactoronthegrowthofhumanerythroidandmyeloidcd34progenitors AT ngachard effectofplateletactivatingfactoronthegrowthofhumanerythroidandmyeloidcd34progenitors AT aallegraud effectofplateletactivatingfactoronthegrowthofhumanerythroidandmyeloidcd34progenitors AT cdulery effectofplateletactivatingfactoronthegrowthofhumanerythroidandmyeloidcd34progenitors AT vpraloran effectofplateletactivatingfactoronthegrowthofhumanerythroidandmyeloidcd34progenitors AT ydenizot effectofplateletactivatingfactoronthegrowthofhumanerythroidandmyeloidcd34progenitors |