Image-based analysis of living mammalian cells using label-free 3D refractive index maps reveals new organelle dynamics and dry mass flux.

Holo-tomographic microscopy (HTM) is a label-free microscopy method reporting the fine changes of a cell's refractive indices (RIs) in three dimensions at high spatial and temporal resolution. By combining HTM with epifluorescence, we demonstrate that mammalian cellular organelles such as lipid...

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Bibliographic Details
Main Authors: Patrick A Sandoz, Christopher Tremblay, F Gisou van der Goot, Mathieu Frechin
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2019-12-01
Series:PLoS Biology
Online Access:https://journals.plos.org/plosbiology/article/file?id=10.1371/journal.pbio.3000553&type=printable
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