Effect of Vitamin C on Cadmium Depending Growth and Vitamin C Contents of Citrobacter Freundii
Citrobacter freundii bacteria were grown in LB medium containing cadmium ion. In order to reduce adverse effect caused by cadmium, different concentrations of vitamin C, were added to the growth medium. While the concentration of bacteria grown was determined by spectrophotometer, the amounts of wat...
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Çanakkale Onsekiz Mart University
2021-09-01
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| Series: | Journal of Advanced Research in Natural and Applied Sciences |
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| Online Access: | https://dergipark.org.tr/en/download/article-file/1813403 |
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| author | Fikret Karataş Muhammad Salihu Ibrahim Meltem Çakmak Dursun Özer Sinan Saydam |
| author_facet | Fikret Karataş Muhammad Salihu Ibrahim Meltem Çakmak Dursun Özer Sinan Saydam |
| author_sort | Fikret Karataş |
| collection | DOAJ |
| description | Citrobacter freundii bacteria were grown in LB medium containing cadmium ion. In order to reduce adverse effect caused by cadmium, different concentrations of vitamin C, were added to the growth medium. While the concentration of bacteria grown was determined by spectrophotometer, the amounts of water and fat-soluble vitamins were also determined by HPLC. The cadmium concentration varied from 0 (control), to 10, 20, 40, 60, 75, 100, 125 ppm in LB growth medium. The effect of cadmium has not been observed on the growth of bacteria, up to 40 ppm, on the other hand no significant microorganism growth was observed over 150 ppm cadmium. Significant change of bacteria concentration was observed in the medium containing 75, 100 and 125 ppm Cd. Microorganism concentrations decreased 18.8, 40.5 and 45.5 percent in the growth medium containing 75, 100 and 125 ppm Cadmium respectively. With the addition of 75 ppm vitamin C to the same growth medium, microorganism concentration was decreased about 2.0, 28.0 and 31.0 percent, respectively compared to the control. In addition, fat and water-soluble vitamins in bacteria grown in cadmium-containing medium was decreased in comparison to the control (p<0.05). With the addition of 25, 50 and 75 ppm vitamin C to cadmium-containing medium, fat and water soluble vitamin concentrations were increased. As a result, it can be said that the negative effects of cadmium on bacterial growth and vitamin content can be reversed by the addition of vitamin C in to the growth medium of bacteria. |
| format | Article |
| id | doaj-art-8048e4860f9749a6a2b4d796e4706728 |
| institution | Kabale University |
| issn | 2757-5195 |
| language | English |
| publishDate | 2021-09-01 |
| publisher | Çanakkale Onsekiz Mart University |
| record_format | Article |
| series | Journal of Advanced Research in Natural and Applied Sciences |
| spelling | doaj-art-8048e4860f9749a6a2b4d796e47067282025-02-05T17:58:10ZengÇanakkale Onsekiz Mart UniversityJournal of Advanced Research in Natural and Applied Sciences2757-51952021-09-017333334210.28979/jarnas.949606453Effect of Vitamin C on Cadmium Depending Growth and Vitamin C Contents of Citrobacter FreundiiFikret Karataş0https://orcid.org/0000-0002-0884-027XMuhammad Salihu Ibrahim1https://orcid.org/0000-0002-7535-4140Meltem Çakmak2https://orcid.org/0000-0002-6291-863XDursun Özer3https://orcid.org/0000-0002-7225-8903Sinan Saydam4https://orcid.org/0000-0003-1531-5454FIRAT ÜNİVERSİTESİ, FEN FAKÜLTESİFIRAT ÜNİVERSİTESİFIRAT ÜNİVERSİTESİFIRAT ÜNİVERSİTESİFIRAT ÜNİVERSİTESİCitrobacter freundii bacteria were grown in LB medium containing cadmium ion. In order to reduce adverse effect caused by cadmium, different concentrations of vitamin C, were added to the growth medium. While the concentration of bacteria grown was determined by spectrophotometer, the amounts of water and fat-soluble vitamins were also determined by HPLC. The cadmium concentration varied from 0 (control), to 10, 20, 40, 60, 75, 100, 125 ppm in LB growth medium. The effect of cadmium has not been observed on the growth of bacteria, up to 40 ppm, on the other hand no significant microorganism growth was observed over 150 ppm cadmium. Significant change of bacteria concentration was observed in the medium containing 75, 100 and 125 ppm Cd. Microorganism concentrations decreased 18.8, 40.5 and 45.5 percent in the growth medium containing 75, 100 and 125 ppm Cadmium respectively. With the addition of 75 ppm vitamin C to the same growth medium, microorganism concentration was decreased about 2.0, 28.0 and 31.0 percent, respectively compared to the control. In addition, fat and water-soluble vitamins in bacteria grown in cadmium-containing medium was decreased in comparison to the control (p<0.05). With the addition of 25, 50 and 75 ppm vitamin C to cadmium-containing medium, fat and water soluble vitamin concentrations were increased. As a result, it can be said that the negative effects of cadmium on bacterial growth and vitamin content can be reversed by the addition of vitamin C in to the growth medium of bacteria.https://dergipark.org.tr/en/download/article-file/1813403cadmiumcitrobacter freundiihplcvitamin cvitamins |
| spellingShingle | Fikret Karataş Muhammad Salihu Ibrahim Meltem Çakmak Dursun Özer Sinan Saydam Effect of Vitamin C on Cadmium Depending Growth and Vitamin C Contents of Citrobacter Freundii Journal of Advanced Research in Natural and Applied Sciences cadmium citrobacter freundii hplc vitamin c vitamins |
| title | Effect of Vitamin C on Cadmium Depending Growth and Vitamin C Contents of Citrobacter Freundii |
| title_full | Effect of Vitamin C on Cadmium Depending Growth and Vitamin C Contents of Citrobacter Freundii |
| title_fullStr | Effect of Vitamin C on Cadmium Depending Growth and Vitamin C Contents of Citrobacter Freundii |
| title_full_unstemmed | Effect of Vitamin C on Cadmium Depending Growth and Vitamin C Contents of Citrobacter Freundii |
| title_short | Effect of Vitamin C on Cadmium Depending Growth and Vitamin C Contents of Citrobacter Freundii |
| title_sort | effect of vitamin c on cadmium depending growth and vitamin c contents of citrobacter freundii |
| topic | cadmium citrobacter freundii hplc vitamin c vitamins |
| url | https://dergipark.org.tr/en/download/article-file/1813403 |
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