Genome-wide identification and expression analysis of phytochrome gene family in Aikang58 wheat (Triticum aestivum L.)
Phytochromes are essential photoreceptors in plants that sense red and far-red light, playing a vital role in regulating plant growth and development through light signal transduction. Despite extensive research on phytochromes in model plants like Arabidopsis and rice, they have received relatively...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1520457/full |
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| author | Zhu Yang Zhu Yang Wenjie Kan Wenjie Kan Ziqi Wang Caiguo Tang Yuan Cheng Yuan Cheng Dacheng Wang Dacheng Wang Yameng Gao Lifang Wu Lifang Wu |
| author_facet | Zhu Yang Zhu Yang Wenjie Kan Wenjie Kan Ziqi Wang Caiguo Tang Yuan Cheng Yuan Cheng Dacheng Wang Dacheng Wang Yameng Gao Lifang Wu Lifang Wu |
| author_sort | Zhu Yang |
| collection | DOAJ |
| description | Phytochromes are essential photoreceptors in plants that sense red and far-red light, playing a vital role in regulating plant growth and development through light signal transduction. Despite extensive research on phytochromes in model plants like Arabidopsis and rice, they have received relatively little attention in wheat. In this study, we employed bioinformatics methods to identify eight TaAkPHY genes in the Aikang58 wheat variety. Based on gene structure, conserved domains, and phylogenetic relationships, the TaAkPHY gene family exhibits a high degree of conservation. Synteny analysis revealed the evolutionary history of the PHY genes in Aikang58 and Chinese Spring wheat (Triticum aestivum L.), barley (Hordeum vulgare L.), rice (Oryza sativa L.), maize (Zea mays L.), quinoa (Chenopodium quinoa Willd.), soybean [Glycine max (L.) Merr.], and Arabidopsis [Arabidopsis thaliana (L.) Heynh.]. Among these species, wheat is most closely related to barley, followed by rice and maize. The cis-acting element analysis indicates that the promoter regions of TaAkPHY genes contain a large number of CAT-box, CGTCA-motif, GC-motif, etc., which are mainly involved in plant development, hormone response, and stress response. Gene expression profiling demonstrated that TaAkPHY genes exhibit varying expression levels across different tissues and are induced by various stress conditions and plant hormone treatments. Co-expression network analysis suggested that TaAkPHY genes may specifically regulate downstream genes associated with stress responses, chloroplast development, and circadian rhythms. Additionally, the least absolute shrinkage and selection operator (LASSO) regression algorithm in machine learning was used to screen transcription factors such as bHLH, WRKY, and MYB that influenced the expression of TaAkPHY genes. This method helps to quickly extract key influencing factors from a large amount of complex data. Overall, these findings provide new insights into the role of phytochromes in wheat growth, development, and stress responses, laying a foundation for future research on phytochromes in wheat. |
| format | Article |
| id | doaj-art-800b661cfbbd46879f69ed06c98dd5da |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-800b661cfbbd46879f69ed06c98dd5da2025-01-21T08:36:49ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-01-011510.3389/fpls.2024.15204571520457Genome-wide identification and expression analysis of phytochrome gene family in Aikang58 wheat (Triticum aestivum L.)Zhu Yang0Zhu Yang1Wenjie Kan2Wenjie Kan3Ziqi Wang4Caiguo Tang5Yuan Cheng6Yuan Cheng7Dacheng Wang8Dacheng Wang9Yameng Gao10Lifang Wu11Lifang Wu12Science Island Branch, University of Science and Technology of China, Hefei, Anhui, ChinaThe Center for Ion Beam Bioengineering & Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, ChinaScience Island Branch, University of Science and Technology of China, Hefei, Anhui, ChinaThe Center for Ion Beam Bioengineering & Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, ChinaThe Center for Ion Beam Bioengineering & Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, ChinaThe Center for Ion Beam Bioengineering & Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, ChinaScience Island Branch, University of Science and Technology of China, Hefei, Anhui, ChinaThe Center for Ion Beam Bioengineering & Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, ChinaScience Island Branch, University of Science and Technology of China, Hefei, Anhui, ChinaThe Center for Ion Beam Bioengineering & Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, ChinaThe Center for Ion Beam Bioengineering & Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, ChinaScience Island Branch, University of Science and Technology of China, Hefei, Anhui, ChinaThe Center for Ion Beam Bioengineering & Green Agriculture, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, Anhui, ChinaPhytochromes are essential photoreceptors in plants that sense red and far-red light, playing a vital role in regulating plant growth and development through light signal transduction. Despite extensive research on phytochromes in model plants like Arabidopsis and rice, they have received relatively little attention in wheat. In this study, we employed bioinformatics methods to identify eight TaAkPHY genes in the Aikang58 wheat variety. Based on gene structure, conserved domains, and phylogenetic relationships, the TaAkPHY gene family exhibits a high degree of conservation. Synteny analysis revealed the evolutionary history of the PHY genes in Aikang58 and Chinese Spring wheat (Triticum aestivum L.), barley (Hordeum vulgare L.), rice (Oryza sativa L.), maize (Zea mays L.), quinoa (Chenopodium quinoa Willd.), soybean [Glycine max (L.) Merr.], and Arabidopsis [Arabidopsis thaliana (L.) Heynh.]. Among these species, wheat is most closely related to barley, followed by rice and maize. The cis-acting element analysis indicates that the promoter regions of TaAkPHY genes contain a large number of CAT-box, CGTCA-motif, GC-motif, etc., which are mainly involved in plant development, hormone response, and stress response. Gene expression profiling demonstrated that TaAkPHY genes exhibit varying expression levels across different tissues and are induced by various stress conditions and plant hormone treatments. Co-expression network analysis suggested that TaAkPHY genes may specifically regulate downstream genes associated with stress responses, chloroplast development, and circadian rhythms. Additionally, the least absolute shrinkage and selection operator (LASSO) regression algorithm in machine learning was used to screen transcription factors such as bHLH, WRKY, and MYB that influenced the expression of TaAkPHY genes. This method helps to quickly extract key influencing factors from a large amount of complex data. Overall, these findings provide new insights into the role of phytochromes in wheat growth, development, and stress responses, laying a foundation for future research on phytochromes in wheat.https://www.frontiersin.org/articles/10.3389/fpls.2024.1520457/fullwheatphytochromesexpression profilesabiotic stressesco-expression networkLASSO regression |
| spellingShingle | Zhu Yang Zhu Yang Wenjie Kan Wenjie Kan Ziqi Wang Caiguo Tang Yuan Cheng Yuan Cheng Dacheng Wang Dacheng Wang Yameng Gao Lifang Wu Lifang Wu Genome-wide identification and expression analysis of phytochrome gene family in Aikang58 wheat (Triticum aestivum L.) Frontiers in Plant Science wheat phytochromes expression profiles abiotic stresses co-expression network LASSO regression |
| title | Genome-wide identification and expression analysis of phytochrome gene family in Aikang58 wheat (Triticum aestivum L.) |
| title_full | Genome-wide identification and expression analysis of phytochrome gene family in Aikang58 wheat (Triticum aestivum L.) |
| title_fullStr | Genome-wide identification and expression analysis of phytochrome gene family in Aikang58 wheat (Triticum aestivum L.) |
| title_full_unstemmed | Genome-wide identification and expression analysis of phytochrome gene family in Aikang58 wheat (Triticum aestivum L.) |
| title_short | Genome-wide identification and expression analysis of phytochrome gene family in Aikang58 wheat (Triticum aestivum L.) |
| title_sort | genome wide identification and expression analysis of phytochrome gene family in aikang58 wheat triticum aestivum l |
| topic | wheat phytochromes expression profiles abiotic stresses co-expression network LASSO regression |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1520457/full |
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