Genome-wide distribution of RNA-DNA hybrids identifies RNase H targets in tRNA genes, retrotransposons and mitochondria.

During transcription, the nascent RNA can invade the DNA template, forming extended RNA-DNA duplexes (R-loops). Here we employ ChIP-seq in strains expressing or lacking RNase H to map targets of RNase H activity throughout the budding yeast genome. In wild-type strains, R-loops were readily detected...

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Bibliographic Details
Main Authors: Aziz El Hage, Shaun Webb, Alastair Kerr, David Tollervey
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-10-01
Series:PLoS Genetics
Online Access:https://doi.org/10.1371/journal.pgen.1004716
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