Quantitative Multiple Competitive PCR of HIV-1 DNA in a Single Reaction Tube
A quantitative multiple competitive PCR (QMC-PCR) for determination of DNA copy numbers is described. Four competitive DNA templates for the env region of HIV-1 were constructed with sizes longer (187 and 163 bp) or shorter (122 and 105 bp) than the 142 bp of the wild-type PCR product. Varying amoun...
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| Main Authors: | Klaus Zimmermann, Daniela Schögl, Barbara Plaimauer, Josef W. Mannhalter |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
1996-09-01
|
| Series: | BioTechniques |
| Online Access: | https://www.future-science.com/doi/10.2144/96213st06 |
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