Quantitative Multiple Competitive PCR of HIV-1 DNA in a Single Reaction Tube

A quantitative multiple competitive PCR (QMC-PCR) for determination of DNA copy numbers is described. Four competitive DNA templates for the env region of HIV-1 were constructed with sizes longer (187 and 163 bp) or shorter (122 and 105 bp) than the 142 bp of the wild-type PCR product. Varying amoun...

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Bibliographic Details
Main Authors: Klaus Zimmermann, Daniela Schögl, Barbara Plaimauer, Josef W. Mannhalter
Format: Article
Language:English
Published: Taylor & Francis Group 1996-09-01
Series:BioTechniques
Online Access:https://www.future-science.com/doi/10.2144/96213st06
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