Haspin kinase inhibition dampens pseudorabies virus infection in vitro
Pseudorabies virus (PRV) represents a considerable infectious threat to the swine industry in China and poses potential health risks to humans. However, there is a notable lack of specific antiviral agents aimed at combating PRV. Haspin is involved in histone phosphorylation during mitosis, while th...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-04-01
|
| Series: | Frontiers in Veterinary Science |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2025.1572729/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849715534172520448 |
|---|---|
| author | Lei Tan Lei Tan Lei Tan Yong Yang Xiaojiu Huang Youqing Yuan Kaixin Wang Xiaoye Peng Yiyan He Yijin Wang Lei Lei Yingyi Chen Deyong Duan Naidong Wang Yi Yang Feiyan Dai Cuiqing Huang Aibing Wang |
| author_facet | Lei Tan Lei Tan Lei Tan Yong Yang Xiaojiu Huang Youqing Yuan Kaixin Wang Xiaoye Peng Yiyan He Yijin Wang Lei Lei Yingyi Chen Deyong Duan Naidong Wang Yi Yang Feiyan Dai Cuiqing Huang Aibing Wang |
| author_sort | Lei Tan |
| collection | DOAJ |
| description | Pseudorabies virus (PRV) represents a considerable infectious threat to the swine industry in China and poses potential health risks to humans. However, there is a notable lack of specific antiviral agents aimed at combating PRV. Haspin is involved in histone phosphorylation during mitosis, while the role of swine Haspin in PRV infection has not been previously investigated. In the present study, we demonstrated that Haspin expression was significantly enhanced in response to PRV infection. Overexpression of the haspin gene notably enhanced PRV infection, while genetic inhibition of haspin gene resulted in a substantial reduction in viral infection. Further investigations indicated that the Haspin kinase inhibitor CHR-6494 effectively suppressed PRV infection in a concentration-dependent manner, primarily by inhibiting viral virus replication rather than interfering with the processes of binding, entry, or release. Additionally, treatment with CHR-6494 effectively restricted Herpes simplex virus type 1 infection in Vero cells. Collectively, these findings indicate that Haspin may serve as a novel therapeutic target for the management of infections caused by Alphaherpesvirinae. |
| format | Article |
| id | doaj-art-7df0c19d0afe4989b7625393c2e4c17f |
| institution | DOAJ |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-7df0c19d0afe4989b7625393c2e4c17f2025-08-20T03:13:21ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-04-011210.3389/fvets.2025.15727291572729Haspin kinase inhibition dampens pseudorabies virus infection in vitroLei Tan0Lei Tan1Lei Tan2Yong Yang3Xiaojiu Huang4Youqing Yuan5Kaixin Wang6Xiaoye Peng7Yiyan He8Yijin Wang9Lei Lei10Yingyi Chen11Deyong Duan12Naidong Wang13Yi Yang14Feiyan Dai15Cuiqing Huang16Aibing Wang17College of Animal Science and Technology, Yangtze University, Jingzhou, ChinaFujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan University, Longyan, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaYunnan Sino-Science Gene Technology Co., Ltd., Kunming, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaDepartment of Chemistry, University College London, London, United KingdomCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaCollege of Animal Science and Technology, Yangtze University, Jingzhou, ChinaCollege of Animal Science and Technology, Yangtze University, Jingzhou, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaCollege of Veterinary Medicine, Yunnan Agricultural University, Kunming, ChinaFujian Provincial Key Laboratory for Prevention and Control of Animal Infectious Diseases and Biotechnology, Longyan University, Longyan, ChinaCollege of Veterinary Medicine, Hunan Agricultural University, Changsha, ChinaPseudorabies virus (PRV) represents a considerable infectious threat to the swine industry in China and poses potential health risks to humans. However, there is a notable lack of specific antiviral agents aimed at combating PRV. Haspin is involved in histone phosphorylation during mitosis, while the role of swine Haspin in PRV infection has not been previously investigated. In the present study, we demonstrated that Haspin expression was significantly enhanced in response to PRV infection. Overexpression of the haspin gene notably enhanced PRV infection, while genetic inhibition of haspin gene resulted in a substantial reduction in viral infection. Further investigations indicated that the Haspin kinase inhibitor CHR-6494 effectively suppressed PRV infection in a concentration-dependent manner, primarily by inhibiting viral virus replication rather than interfering with the processes of binding, entry, or release. Additionally, treatment with CHR-6494 effectively restricted Herpes simplex virus type 1 infection in Vero cells. Collectively, these findings indicate that Haspin may serve as a novel therapeutic target for the management of infections caused by Alphaherpesvirinae.https://www.frontiersin.org/articles/10.3389/fvets.2025.1572729/fullpseudorabies virusHaspin kinaseantiviral activityCHR-6494replication stage |
| spellingShingle | Lei Tan Lei Tan Lei Tan Yong Yang Xiaojiu Huang Youqing Yuan Kaixin Wang Xiaoye Peng Yiyan He Yijin Wang Lei Lei Yingyi Chen Deyong Duan Naidong Wang Yi Yang Feiyan Dai Cuiqing Huang Aibing Wang Haspin kinase inhibition dampens pseudorabies virus infection in vitro Frontiers in Veterinary Science pseudorabies virus Haspin kinase antiviral activity CHR-6494 replication stage |
| title | Haspin kinase inhibition dampens pseudorabies virus infection in vitro |
| title_full | Haspin kinase inhibition dampens pseudorabies virus infection in vitro |
| title_fullStr | Haspin kinase inhibition dampens pseudorabies virus infection in vitro |
| title_full_unstemmed | Haspin kinase inhibition dampens pseudorabies virus infection in vitro |
| title_short | Haspin kinase inhibition dampens pseudorabies virus infection in vitro |
| title_sort | haspin kinase inhibition dampens pseudorabies virus infection in vitro |
| topic | pseudorabies virus Haspin kinase antiviral activity CHR-6494 replication stage |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2025.1572729/full |
| work_keys_str_mv | AT leitan haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT leitan haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT leitan haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT yongyang haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT xiaojiuhuang haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT youqingyuan haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT kaixinwang haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT xiaoyepeng haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT yiyanhe haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT yijinwang haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT leilei haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT yingyichen haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT deyongduan haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT naidongwang haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT yiyang haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT feiyandai haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT cuiqinghuang haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro AT aibingwang haspinkinaseinhibitiondampenspseudorabiesvirusinfectioninvitro |