Improved constructs for bait RNA display in a bacterial three-hybrid assay

Improved constructs for bait RNA display in a bacterial three-hybrid assay

Abstract We have previously developed a transcription-based bacterial three-hybrid (B3H) assay as a genetic approach to probe RNA–protein interactions inside of E. coli cells. This system offers a straightforward path to identify and assess the consequences of mutations in RBPs with molecular phenot...

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Bibliographic Details
Main Authors: Linh D. Nguyen, Hannah LeBlanc, Katherine E. Berry
Format: Article
Language:English
Published: Nature Portfolio 2025-01-01
Series:Scientific Reports
Subjects:
RNA–protein interactions
Hfq
Bacterial sRNAs
B3H assay
GC clamps
Online Access:https://doi.org/10.1038/s41598-024-85082-9
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