Improved constructs for bait RNA display in a bacterial three-hybrid assay
Abstract We have previously developed a transcription-based bacterial three-hybrid (B3H) assay as a genetic approach to probe RNA–protein interactions inside of E. coli cells. This system offers a straightforward path to identify and assess the consequences of mutations in RBPs with molecular phenot...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-01-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-024-85082-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|