A new statistical metric for robust target detection in cryo-EM using 2D template matching

2D template matching (2DTM) can be used to detect molecules and their assemblies in cellular cryo-EM images with high positional and orientational accuracy. While 2DTM successfully detects spherical targets such as large ribosomal subunits, challenges remain in detecting smaller and more aspherical...

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Bibliographic Details
Main Authors: Kexin Zhang, Pilar Cossio, Aaditya V. Rangan, Bronwyn A. Lucas, Nikolaus Grigorieff
Format: Article
Language:English
Published: International Union of Crystallography 2025-03-01
Series:IUCrJ
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Online Access:https://journals.iucr.org/paper?S2052252524011771
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