Protocol for dual metabolomics and proteomics using nanoflow liquid chromatography-tandem mass spectrometry

Protocol for dual metabolomics and proteomics using nanoflow liquid chromatography-tandem mass spectrometry

Summary: Nanoflow liquid chromatography-tandem mass spectrometry (nLC-MS) benefits untargeted metabolomics by enhancing sensitivity and integrating proteomics for the same sample. Here, we present a protocol to enable nLC-MS for dual metabolomics and proteomics. We describe steps for solid-phase mic...

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Bibliographic Details
Main Authors: Weiwei Lin, Fatemeh Mousavi, Benjamin C. Blum, Christian F. Heckendorf, Matthew Lawton, Noah Lampl, Ryan Hekman, Mark McComb, Andrew Emili
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:STAR Protocols
Subjects:
Bioinformatics
Mass Spectrometry
Metabolomics
Proteomics
Systems biology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725001510
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http://www.sciencedirect.com/science/article/pii/S2666166725001510

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