Validation and implementation of TaqMAMA RT-PCR for SARS-CoV-2 variant surveillance: experience from a high-volume setting

Validation and implementation of TaqMAMA RT-PCR for SARS-CoV-2 variant surveillance: experience from a high-volume setting

Abstract Background The genomic surveillance of SARS-CoV-2 is challenging in high-volume, resource-limited settings. Faster and less expensive methods are required for the prompt detection of variants of interest. This study aimed to validate and implement the TaqMAMA RT-PCR method for the detection...

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Main Authors: José Nicolas Aguirre-Pineda, Mario Alberto Mújica-Sánchez, Hansel Hugo Chávez-Morales, Gabriel Cojuc-Konigsberg, Alan Braverman-Poyastro, Alberto Moscona-Nissan, Gastón Becherano-Razon, Alberto Guijosa, Damilda Duarte, Maria Del Carmen García-Colín, Martha Angella Durán-Barrón, Eduardo Becerril-Vargas
Format: Article
Language:English
Published: BMC 2025-02-01
Series:BMC Infectious Diseases
Subjects:
RT-PCR
TaqMAMA
SARS-CoV-2
Genomic surveillance
Variants of interest
Whole genome sequencing
Online Access:https://doi.org/10.1186/s12879-025-10645-8
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https://doi.org/10.1186/s12879-025-10645-8

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