Unfolding of viral protein 1 N-termini facilitates genome ejection from recombinant adeno-associated virus serotype 8

Unfolding of viral protein 1 N-termini facilitates genome ejection from recombinant adeno-associated virus serotype 8

The role of viral protein (VP) 1 and VP2, which comprise the recombinant adeno-associated virus (rAAV) capsid, in heat-induced genome release was investigated using rAAV serotype 8 (rAAV8) samples with a high VP1/VP2 to VP3 ratio, a low VP1/VP2 to VP3 ratio, and VP3 only. The thermal unfolding of th...

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Main Authors: Yuki Yamaguchi, Saki Shimojo, Tomohiko Ikeda, Mitsuko Fukuhara, Yasuo Tsunaka, Risa Shibuya, Mark Allen Vergara Rocafort, Ryoji Nakatsuka, Kiichi Hirohata, Tetsuo Torisu, Susumu Uchiyama
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
gene therapy
adeno-associated virus
genome release
thermal unfolding
mass photometry
nano-differential scanning fluorimetry
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050125000750
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