Effect of Stretching Force on the Cells of Epithelial Rests of Malassez In Vitro
Background and Objective. The aim of this study was to investigate the behavior of cells from epithelial rest of Malassez (ERM) against stretching force. Material and Methods. ERM-cultured cells were stretched for 1 hour, at the cycle of 18% elongation for 1 second followed by 1-second relaxation. T...
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| Format: | Article |
| Language: | English |
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Wiley
2010-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2010/458408 |
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| _version_ | 1832567178060103680 |
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| author | Teruyoshi Koshihara Kenichi Matsuzaka Toru Sato Takashi Inoue |
| author_facet | Teruyoshi Koshihara Kenichi Matsuzaka Toru Sato Takashi Inoue |
| author_sort | Teruyoshi Koshihara |
| collection | DOAJ |
| description | Background and Objective. The aim of this study was to investigate the behavior of cells from epithelial rest of Malassez (ERM) against stretching force. Material and Methods. ERM-cultured cells were stretched for 1 hour, at the cycle of 18% elongation for 1 second followed by 1-second relaxation. The cells without addition of stretching force were used as controls. The cells were observed by immunohistochmical staining using actin 0, 12, 24, 36, 48, and 72 hours. Furthermore, expressions of HSP70-, VEGF-, and OPN-mRNAs of cells were also evaluated using quantitative RT-PCR. Results. Actin filaments were randomly orientated in the cytoplasm in the control group, whereas in the stretching group, actin filaments were orientated comparatively parallel to the stretching direction. Expression of HSP70-mRNA in the stretching group was significantly higher than that of control group at 12, 24, 36 hours (P<.05). Expression of VEGF-mRNA in the stretching group was significantly higher than that of control group at 24, 36, 48, and 72 hours (P<.05). Expression of OPN-mRNA in the stretching group was significantly higher than that of control group at 12 and 24 hours (P<.05). Conclusion. ERM cells response against the stretching force by expressing HSP70, VEGF, and OPN. |
| format | Article |
| id | doaj-art-7c6c0e5e3e7d4a4e9059e5f95271b413 |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2010-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-7c6c0e5e3e7d4a4e9059e5f95271b4132025-02-03T01:02:08ZengWileyInternational Journal of Dentistry1687-87281687-87362010-01-01201010.1155/2010/458408458408Effect of Stretching Force on the Cells of Epithelial Rests of Malassez In VitroTeruyoshi Koshihara0Kenichi Matsuzaka1Toru Sato2Takashi Inoue3Department of Crown & Bridge Prosthodontics, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba 261-8502, JapanOral Health Science Center hrc7, Department of Clinical Pathophysiology, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba 261-8502, JapanDepartment of Crown & Bridge Prosthodontics, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba 261-8502, JapanOral Health Science Center hrc7, Department of Clinical Pathophysiology, Tokyo Dental College, 1-2-2, Masago, Mihama-ku, Chiba 261-8502, JapanBackground and Objective. The aim of this study was to investigate the behavior of cells from epithelial rest of Malassez (ERM) against stretching force. Material and Methods. ERM-cultured cells were stretched for 1 hour, at the cycle of 18% elongation for 1 second followed by 1-second relaxation. The cells without addition of stretching force were used as controls. The cells were observed by immunohistochmical staining using actin 0, 12, 24, 36, 48, and 72 hours. Furthermore, expressions of HSP70-, VEGF-, and OPN-mRNAs of cells were also evaluated using quantitative RT-PCR. Results. Actin filaments were randomly orientated in the cytoplasm in the control group, whereas in the stretching group, actin filaments were orientated comparatively parallel to the stretching direction. Expression of HSP70-mRNA in the stretching group was significantly higher than that of control group at 12, 24, 36 hours (P<.05). Expression of VEGF-mRNA in the stretching group was significantly higher than that of control group at 24, 36, 48, and 72 hours (P<.05). Expression of OPN-mRNA in the stretching group was significantly higher than that of control group at 12 and 24 hours (P<.05). Conclusion. ERM cells response against the stretching force by expressing HSP70, VEGF, and OPN.http://dx.doi.org/10.1155/2010/458408 |
| spellingShingle | Teruyoshi Koshihara Kenichi Matsuzaka Toru Sato Takashi Inoue Effect of Stretching Force on the Cells of Epithelial Rests of Malassez In Vitro International Journal of Dentistry |
| title | Effect of Stretching Force on the Cells of Epithelial Rests of Malassez In Vitro |
| title_full | Effect of Stretching Force on the Cells of Epithelial Rests of Malassez In Vitro |
| title_fullStr | Effect of Stretching Force on the Cells of Epithelial Rests of Malassez In Vitro |
| title_full_unstemmed | Effect of Stretching Force on the Cells of Epithelial Rests of Malassez In Vitro |
| title_short | Effect of Stretching Force on the Cells of Epithelial Rests of Malassez In Vitro |
| title_sort | effect of stretching force on the cells of epithelial rests of malassez in vitro |
| url | http://dx.doi.org/10.1155/2010/458408 |
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