Impact of Edible Insect Polysaccharides on Mouse Gut Microbiota: A Study on White-Spotted Flower Chafer Larva (<i>Protaetia brevitarsis seulensis</i>) and Silkworm Pupa (<i>Bombyx mori</i>)
The increasing global population and the environmental consequences of meat consumption have led to the exploration of alternative sources of protein. Edible insects have gained attention as a sustainable and nutritionally rich meat alternative. We investigated the effects of two commonly consumed i...
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2024-12-01
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| author | Joon-Ha Lee Hyojung Son Sathiyamoorthy Subramaniyam Hyun-Jung Lim Sohyun Park Ra-Yeong Choi In-Woo Kim Minchul Seo Hae-Yong Kweon Yongsoon Kim Seong-Wan Kim Jong-Soon Choi Younhee Shin |
| author_facet | Joon-Ha Lee Hyojung Son Sathiyamoorthy Subramaniyam Hyun-Jung Lim Sohyun Park Ra-Yeong Choi In-Woo Kim Minchul Seo Hae-Yong Kweon Yongsoon Kim Seong-Wan Kim Jong-Soon Choi Younhee Shin |
| author_sort | Joon-Ha Lee |
| collection | DOAJ |
| description | The increasing global population and the environmental consequences of meat consumption have led to the exploration of alternative sources of protein. Edible insects have gained attention as a sustainable and nutritionally rich meat alternative. We investigated the effects of two commonly consumed insects, <i>Protaetia brevitarsis seulensis</i> larva and <i>Bombyx mori</i> pupa, on beneficial gut microbiota growth, using whole 16s metagenome sequencing to assess diet-associated changes. Seven-week-old female C57BL/6J mice were administered the edible insects, along with fracto-oligosaccharide (FOS) as a positive control and sham (phosphate buffer saline (PBS)) as a negative control, to assess the relative abundance of insect-diet-associated gut microbes. In total, 567 genera and 470 species were observed, and among these, 15 bacterial genera were differentially abundant in all three groups. These results show that among the two insects, <i>Bombyx mori</i> pupa polysaccharides have a greater ability to regulate beneficial probiotics and next-generation probiotics. In particular, <i>Lactococcus garvieae</i>, which has promising effects on the gastrointestinal tracts of humans and animals, was significantly enriched in both <i>Protaetia brevitarsis seulensis</i> larva and <i>Bombyx mori</i> pupa polysaccharides, similar to fracto-oligosaccharide. The results suggest that the consumption of these insects, particularly polysaccharides, can enhance the growth of beneficial gut microbes, potentially leading to improved overall health in healthy populations. |
| format | Article |
| id | doaj-art-7c6aed1af23040558e3cca9833d08036 |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-7c6aed1af23040558e3cca9833d080362025-01-10T13:17:29ZengMDPI AGFoods2304-81582024-12-01141610.3390/foods14010006Impact of Edible Insect Polysaccharides on Mouse Gut Microbiota: A Study on White-Spotted Flower Chafer Larva (<i>Protaetia brevitarsis seulensis</i>) and Silkworm Pupa (<i>Bombyx mori</i>)Joon-Ha Lee0Hyojung Son1Sathiyamoorthy Subramaniyam2Hyun-Jung Lim3Sohyun Park4Ra-Yeong Choi5In-Woo Kim6Minchul Seo7Hae-Yong Kweon8Yongsoon Kim9Seong-Wan Kim10Jong-Soon Choi11Younhee Shin12Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of KoreaResearch and Development Center, Insilicogen Inc., Yongin 16954, Republic of KoreaResearch and Development Center, Insilicogen Inc., Yongin 16954, Republic of KoreaDepartment of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of KoreaDepartment of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of KoreaDepartment of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of KoreaDepartment of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of KoreaDepartment of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of KoreaDepartment of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of KoreaDepartment of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of KoreaDepartment of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration, Wanju 55365, Republic of KoreaDepartment of Family Medicine, College of Medicine, Kosin University, Busan 49267, Republic of KoreaResearch and Development Center, Insilicogen Inc., Yongin 16954, Republic of KoreaThe increasing global population and the environmental consequences of meat consumption have led to the exploration of alternative sources of protein. Edible insects have gained attention as a sustainable and nutritionally rich meat alternative. We investigated the effects of two commonly consumed insects, <i>Protaetia brevitarsis seulensis</i> larva and <i>Bombyx mori</i> pupa, on beneficial gut microbiota growth, using whole 16s metagenome sequencing to assess diet-associated changes. Seven-week-old female C57BL/6J mice were administered the edible insects, along with fracto-oligosaccharide (FOS) as a positive control and sham (phosphate buffer saline (PBS)) as a negative control, to assess the relative abundance of insect-diet-associated gut microbes. In total, 567 genera and 470 species were observed, and among these, 15 bacterial genera were differentially abundant in all three groups. These results show that among the two insects, <i>Bombyx mori</i> pupa polysaccharides have a greater ability to regulate beneficial probiotics and next-generation probiotics. In particular, <i>Lactococcus garvieae</i>, which has promising effects on the gastrointestinal tracts of humans and animals, was significantly enriched in both <i>Protaetia brevitarsis seulensis</i> larva and <i>Bombyx mori</i> pupa polysaccharides, similar to fracto-oligosaccharide. The results suggest that the consumption of these insects, particularly polysaccharides, can enhance the growth of beneficial gut microbes, potentially leading to improved overall health in healthy populations.https://www.mdpi.com/2304-8158/14/1/6entomophagyfood safetygut microbiomeinsect polysaccharideprebioticsprobiotics |
| spellingShingle | Joon-Ha Lee Hyojung Son Sathiyamoorthy Subramaniyam Hyun-Jung Lim Sohyun Park Ra-Yeong Choi In-Woo Kim Minchul Seo Hae-Yong Kweon Yongsoon Kim Seong-Wan Kim Jong-Soon Choi Younhee Shin Impact of Edible Insect Polysaccharides on Mouse Gut Microbiota: A Study on White-Spotted Flower Chafer Larva (<i>Protaetia brevitarsis seulensis</i>) and Silkworm Pupa (<i>Bombyx mori</i>) Foods entomophagy food safety gut microbiome insect polysaccharide prebiotics probiotics |
| title | Impact of Edible Insect Polysaccharides on Mouse Gut Microbiota: A Study on White-Spotted Flower Chafer Larva (<i>Protaetia brevitarsis seulensis</i>) and Silkworm Pupa (<i>Bombyx mori</i>) |
| title_full | Impact of Edible Insect Polysaccharides on Mouse Gut Microbiota: A Study on White-Spotted Flower Chafer Larva (<i>Protaetia brevitarsis seulensis</i>) and Silkworm Pupa (<i>Bombyx mori</i>) |
| title_fullStr | Impact of Edible Insect Polysaccharides on Mouse Gut Microbiota: A Study on White-Spotted Flower Chafer Larva (<i>Protaetia brevitarsis seulensis</i>) and Silkworm Pupa (<i>Bombyx mori</i>) |
| title_full_unstemmed | Impact of Edible Insect Polysaccharides on Mouse Gut Microbiota: A Study on White-Spotted Flower Chafer Larva (<i>Protaetia brevitarsis seulensis</i>) and Silkworm Pupa (<i>Bombyx mori</i>) |
| title_short | Impact of Edible Insect Polysaccharides on Mouse Gut Microbiota: A Study on White-Spotted Flower Chafer Larva (<i>Protaetia brevitarsis seulensis</i>) and Silkworm Pupa (<i>Bombyx mori</i>) |
| title_sort | impact of edible insect polysaccharides on mouse gut microbiota a study on white spotted flower chafer larva i protaetia brevitarsis seulensis i and silkworm pupa i bombyx mori i |
| topic | entomophagy food safety gut microbiome insect polysaccharide prebiotics probiotics |
| url | https://www.mdpi.com/2304-8158/14/1/6 |
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