Protocol for automated graph-based clustering of single-cell RNA-seq data with application in mouse intestinal stem cells

Protocol for automated graph-based clustering of single-cell RNA-seq data with application in mouse intestinal stem cells

Summary: We present a protocol for isolating highly purified crypt epithelial cells from the mouse intestine for single-cell RNA sequencing (scRNA-seq). Optimized for the mouse jejunum, it can be adapted to all intestinal tracts, including the colon. The pipeline incorporates automated community det...

Full description

Saved in:
Bibliographic Details
Main Authors: Alexander L.E. Wang, Luca Zanella, Yosuke Ochiai, Leonardo Golinelli, Andrea Califano, Ermanno Malagola, Alessandro Vasciaveo
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Bioinformatics
Cell Biology
Cell Differentiation
Cell isolation
Flow Cytometry
Genomics
Online Access:http://www.sciencedirect.com/science/article/pii/S266616672500406X
Tags: Add Tag
No Tags, Be the first to tag this record!

Internet

http://www.sciencedirect.com/science/article/pii/S266616672500406X

Similar Items