Reference genes validation in tear fluid for RNA analysis in ocular surface disease

Reference genes validation in tear fluid for RNA analysis in ocular surface disease

Abstract Proper normalization of gene expression data is essential for detecting early molecular alterations in eye disorders. In this study, we assessed seven potential reference genes (18S, RER1, ACTB, GAPDH, PGK1, UBC, and AP3D1) in tear fluid collected from individuals with dry eye disease and g...

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Bibliographic Details
Main Authors: Diego A. Ojeda, Hooman Ahmadzadeh, Sarp Orgul, Angela Gomez Bedoya, Swati Gupta, Katherine Held, Swarup S. Swaminathan, Alfonso L. Sabater
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
Subjects:
Tear fluid
Reference genes
Gene expression normalization
Dry eye disease
Glaucoma
Inflammasome activation
Online Access:https://doi.org/10.1038/s41598-025-05638-1
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https://doi.org/10.1038/s41598-025-05638-1

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