Multienzyme cascade for synthesis of hydroxytyrosol via engineered Escherichia coli
Abstract Hydroxytyrosol, a fine chemical, is widely utilized in food and pharmaceutical industries. In this study, we constructed a pathway to produce hydroxytyrosol by co-expressing tyrosin-phenol lyase (TPL), L-amino acid dehydrogenase (aadL), α-keto acid decarboxylase (KAD), aldehyde reductase (y...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-84624-5 |
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| author | Tianzhen Xiong Xinmeng Li Wei Liu Huidie Yue Junling Liu Dingyuan Bai Wei Li Guangyan Fan |
| author_facet | Tianzhen Xiong Xinmeng Li Wei Liu Huidie Yue Junling Liu Dingyuan Bai Wei Li Guangyan Fan |
| author_sort | Tianzhen Xiong |
| collection | DOAJ |
| description | Abstract Hydroxytyrosol, a fine chemical, is widely utilized in food and pharmaceutical industries. In this study, we constructed a pathway to produce hydroxytyrosol by co-expressing tyrosin-phenol lyase (TPL), L-amino acid dehydrogenase (aadL), α-keto acid decarboxylase (KAD), aldehyde reductase (yahK) and glucose dehydrogenase (gdh). We changed combinations between plasmids with different copy numbers and target genes, resulting in 84% increase in hydroxytyrosol production. The yield of hydroxytyrosol was further increased 89.3% by optimizing the temperature and pH. Finally, 55.3 mM hydroxytyrosol was produced within 14 h by fed-batch biotransformation. This study provides a novel approach for hydroxytyrosol production. |
| format | Article |
| id | doaj-art-7bf47703618f444cb087375ce49d0f34 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-7bf47703618f444cb087375ce49d0f342025-01-05T12:13:30ZengNature PortfolioScientific Reports2045-23222025-01-011511810.1038/s41598-024-84624-5Multienzyme cascade for synthesis of hydroxytyrosol via engineered Escherichia coliTianzhen Xiong0Xinmeng Li1Wei Liu2Huidie Yue3Junling Liu4Dingyuan Bai5Wei Li6Guangyan Fan7Dabie Mountain Laboratory, College of Tea and Food Science, Xinyang Normal UniversityDabie Mountain Laboratory, College of Tea and Food Science, Xinyang Normal UniversityInstitute of Agricultural Quality Standards and Testing Technology, Jilin Academy of Agricultural SciencesDabie Mountain Laboratory, College of Tea and Food Science, Xinyang Normal UniversityDabie Mountain Laboratory, College of Tea and Food Science, Xinyang Normal UniversityDabie Mountain Laboratory, College of Tea and Food Science, Xinyang Normal UniversityDabie Mountain Laboratory, College of Tea and Food Science, Xinyang Normal UniversityDabie Mountain Laboratory, College of Tea and Food Science, Xinyang Normal UniversityAbstract Hydroxytyrosol, a fine chemical, is widely utilized in food and pharmaceutical industries. In this study, we constructed a pathway to produce hydroxytyrosol by co-expressing tyrosin-phenol lyase (TPL), L-amino acid dehydrogenase (aadL), α-keto acid decarboxylase (KAD), aldehyde reductase (yahK) and glucose dehydrogenase (gdh). We changed combinations between plasmids with different copy numbers and target genes, resulting in 84% increase in hydroxytyrosol production. The yield of hydroxytyrosol was further increased 89.3% by optimizing the temperature and pH. Finally, 55.3 mM hydroxytyrosol was produced within 14 h by fed-batch biotransformation. This study provides a novel approach for hydroxytyrosol production.https://doi.org/10.1038/s41598-024-84624-5HydroxytyrosolEscherichia coliCascade reactionWhole cell biotransformation |
| spellingShingle | Tianzhen Xiong Xinmeng Li Wei Liu Huidie Yue Junling Liu Dingyuan Bai Wei Li Guangyan Fan Multienzyme cascade for synthesis of hydroxytyrosol via engineered Escherichia coli Scientific Reports Hydroxytyrosol Escherichia coli Cascade reaction Whole cell biotransformation |
| title | Multienzyme cascade for synthesis of hydroxytyrosol via engineered Escherichia coli |
| title_full | Multienzyme cascade for synthesis of hydroxytyrosol via engineered Escherichia coli |
| title_fullStr | Multienzyme cascade for synthesis of hydroxytyrosol via engineered Escherichia coli |
| title_full_unstemmed | Multienzyme cascade for synthesis of hydroxytyrosol via engineered Escherichia coli |
| title_short | Multienzyme cascade for synthesis of hydroxytyrosol via engineered Escherichia coli |
| title_sort | multienzyme cascade for synthesis of hydroxytyrosol via engineered escherichia coli |
| topic | Hydroxytyrosol Escherichia coli Cascade reaction Whole cell biotransformation |
| url | https://doi.org/10.1038/s41598-024-84624-5 |
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