Development of novel neutralizing single-chain fragment variable antibodies against S100A8
Abstract S100A8 plays a critical role in the pathogenesis of several inflammatory diseases and multiple types of cancer. Therefore, targeting the S100A8 function may alleviate the pathogenic process in various diseases. Here, specific single-chain variable fragment (scFv) antibodies targeting recomb...
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Nature Portfolio
2025-04-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-96211-3 |
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| author | Hamideh Raeisi Masoumeh Azimirad Alexandru Schiopu Amir-Hassan Zarnani Hamid Asadzadeh Aghdaei Elham Abdemohamadi Mohammad Reza Zali Abbas Yadegar |
| author_facet | Hamideh Raeisi Masoumeh Azimirad Alexandru Schiopu Amir-Hassan Zarnani Hamid Asadzadeh Aghdaei Elham Abdemohamadi Mohammad Reza Zali Abbas Yadegar |
| author_sort | Hamideh Raeisi |
| collection | DOAJ |
| description | Abstract S100A8 plays a critical role in the pathogenesis of several inflammatory diseases and multiple types of cancer. Therefore, targeting the S100A8 function may alleviate the pathogenic process in various diseases. Here, specific single-chain variable fragment (scFv) antibodies targeting recombinant S100A8 (rS100A8) were selected by phage display technique and characterized using cDNA sequencing, immunoassay tests, and molecular docking. The neutralizing activity of scFvs was examined by a cell viability assay in rS100A8-treated macrophages. Furthermore, the modulatory effects of scFvs on the expression of inflammatory markers and apoptosis-related genes in macrophages and human colorectal carcinoma HT-29 cells treated with rS100A8 or dextran sulfate sodium (DSS) were assessed by RT-qPCR and flow cytometry. Based on our results, four scFvs were identified to be capable of detecting rS100A8 in the immunoassay tests. Among the selected scFvs, two clones (SA8-E6 and SA8-E12), alone or in combination, exhibited the highest blocking activity on rS100A8 and potently inhibited S100A8-induced cytotoxicity in macrophages. The use of a SA8-E6 and SA8-E12 (SA8-E6-12) cocktail inhibited the upregulation of TLR4 and RAGE, as well as inflammation and apoptosis-related genes in macrophages and HT-29 cells stimulated with rS100A8. Additionally, SA8-E6-12 exerted a significant inhibitory effect on inflammation and apoptosis induced by the S100A8/A9 complex in DSS-stimulated macrophages. We also demonstrated by molecular docking that the interaction of S100A8 with SA8-E6-12 was mainly restricted to the binding domain involved in the connection of S100A8 to TLR4. Further studies are required to explore the efficacy of these anti-S100A8 scFvs as potential therapeutic agents using in vivo models of S100A8-driven inflammatory diseases. |
| format | Article |
| id | doaj-art-7bdab1949a2843a8a7dbe36037f0ab39 |
| institution | OA Journals |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-7bdab1949a2843a8a7dbe36037f0ab392025-08-20T02:17:09ZengNature PortfolioScientific Reports2045-23222025-04-0115111910.1038/s41598-025-96211-3Development of novel neutralizing single-chain fragment variable antibodies against S100A8Hamideh Raeisi0Masoumeh Azimirad1Alexandru Schiopu2Amir-Hassan Zarnani3Hamid Asadzadeh Aghdaei4Elham Abdemohamadi5Mohammad Reza Zali6Abbas Yadegar7Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical SciencesFoodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical SciencesDepartment of Translational Medicine, Lund UniversityDepartment of Immunology, School of Public Health, Tehran University of Medical Sciences Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical SciencesFoodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical SciencesGastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical SciencesFoodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical SciencesAbstract S100A8 plays a critical role in the pathogenesis of several inflammatory diseases and multiple types of cancer. Therefore, targeting the S100A8 function may alleviate the pathogenic process in various diseases. Here, specific single-chain variable fragment (scFv) antibodies targeting recombinant S100A8 (rS100A8) were selected by phage display technique and characterized using cDNA sequencing, immunoassay tests, and molecular docking. The neutralizing activity of scFvs was examined by a cell viability assay in rS100A8-treated macrophages. Furthermore, the modulatory effects of scFvs on the expression of inflammatory markers and apoptosis-related genes in macrophages and human colorectal carcinoma HT-29 cells treated with rS100A8 or dextran sulfate sodium (DSS) were assessed by RT-qPCR and flow cytometry. Based on our results, four scFvs were identified to be capable of detecting rS100A8 in the immunoassay tests. Among the selected scFvs, two clones (SA8-E6 and SA8-E12), alone or in combination, exhibited the highest blocking activity on rS100A8 and potently inhibited S100A8-induced cytotoxicity in macrophages. The use of a SA8-E6 and SA8-E12 (SA8-E6-12) cocktail inhibited the upregulation of TLR4 and RAGE, as well as inflammation and apoptosis-related genes in macrophages and HT-29 cells stimulated with rS100A8. Additionally, SA8-E6-12 exerted a significant inhibitory effect on inflammation and apoptosis induced by the S100A8/A9 complex in DSS-stimulated macrophages. We also demonstrated by molecular docking that the interaction of S100A8 with SA8-E6-12 was mainly restricted to the binding domain involved in the connection of S100A8 to TLR4. Further studies are required to explore the efficacy of these anti-S100A8 scFvs as potential therapeutic agents using in vivo models of S100A8-driven inflammatory diseases.https://doi.org/10.1038/s41598-025-96211-3S100A8scFvPhage displayInflammationApoptosisTherapeutics |
| spellingShingle | Hamideh Raeisi Masoumeh Azimirad Alexandru Schiopu Amir-Hassan Zarnani Hamid Asadzadeh Aghdaei Elham Abdemohamadi Mohammad Reza Zali Abbas Yadegar Development of novel neutralizing single-chain fragment variable antibodies against S100A8 Scientific Reports S100A8 scFv Phage display Inflammation Apoptosis Therapeutics |
| title | Development of novel neutralizing single-chain fragment variable antibodies against S100A8 |
| title_full | Development of novel neutralizing single-chain fragment variable antibodies against S100A8 |
| title_fullStr | Development of novel neutralizing single-chain fragment variable antibodies against S100A8 |
| title_full_unstemmed | Development of novel neutralizing single-chain fragment variable antibodies against S100A8 |
| title_short | Development of novel neutralizing single-chain fragment variable antibodies against S100A8 |
| title_sort | development of novel neutralizing single chain fragment variable antibodies against s100a8 |
| topic | S100A8 scFv Phage display Inflammation Apoptosis Therapeutics |
| url | https://doi.org/10.1038/s41598-025-96211-3 |
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