Annexin-A1 deficiency uncovers female-specific pathways in blood pressure control and cardiovascular remodeling in mice

Abstract Cardiovascular disease exhibits distinct sex-based differences, yet the mechanisms underlying these differences remain under-explored. The pro-resolving mediator annexin-A1 (ANXA1) is a pivotal regulator in inflammation resolution and tissue homeostasis, including within the cardiovascular...

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Main Authors: Jaideep Singh, Kristy L. Jackson, Haoyun Fang, Feng Shii Tang, Cindy Gueguen, Alex M. Parker, HsinErh Chen, Cameron J. Nowell, Helen Kiriazis, Ekaterina Salimova, Owen L. Woodman, Rebecca H. Ritchie, Geoffrey A. Head, David W. Greening, Cheng Xue Qin
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Communications Biology
Online Access:https://doi.org/10.1038/s42003-025-08291-6
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author Jaideep Singh
Kristy L. Jackson
Haoyun Fang
Feng Shii Tang
Cindy Gueguen
Alex M. Parker
HsinErh Chen
Cameron J. Nowell
Helen Kiriazis
Ekaterina Salimova
Owen L. Woodman
Rebecca H. Ritchie
Geoffrey A. Head
David W. Greening
Cheng Xue Qin
author_facet Jaideep Singh
Kristy L. Jackson
Haoyun Fang
Feng Shii Tang
Cindy Gueguen
Alex M. Parker
HsinErh Chen
Cameron J. Nowell
Helen Kiriazis
Ekaterina Salimova
Owen L. Woodman
Rebecca H. Ritchie
Geoffrey A. Head
David W. Greening
Cheng Xue Qin
author_sort Jaideep Singh
collection DOAJ
description Abstract Cardiovascular disease exhibits distinct sex-based differences, yet the mechanisms underlying these differences remain under-explored. The pro-resolving mediator annexin-A1 (ANXA1) is a pivotal regulator in inflammation resolution and tissue homeostasis, including within the cardiovascular system. However, the sex-specific differences in ANXA1 in blood pressure regulation have not been investigated. Here, we demonstrate that deficiency of ANXA1 exacerbates angiotensin II-induced adverse aortic and cardiac structural remodeling, mitochondrial proteome dysregulation, and impaired mitochondrial function in preclinical hypertensive models, exacerbated in females. Mechanistically, we demonstrate that estrogen upregulates ANXA1 levels, associated with dysregulation of inflammatory and mitochondrial networks, suggesting that the estrogen-ANXA1 axis plays a critical role in modulating inflammation and preventing pathological remodeling. In conclusion, this study advances the understanding of female-specific cardiac and aortic tissue and cellular alterations in hypertension, providing a platform for developing therapeutic ANXA1 mimetics that address the unique pathophysiological features of hypertension in females.
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spelling doaj-art-7bab199dba18426fab9f206df8dcff132025-08-20T04:01:41ZengNature PortfolioCommunications Biology2399-36422025-07-018111610.1038/s42003-025-08291-6Annexin-A1 deficiency uncovers female-specific pathways in blood pressure control and cardiovascular remodeling in miceJaideep Singh0Kristy L. Jackson1Haoyun Fang2Feng Shii Tang3Cindy Gueguen4Alex M. Parker5HsinErh Chen6Cameron J. Nowell7Helen Kiriazis8Ekaterina Salimova9Owen L. Woodman10Rebecca H. Ritchie11Geoffrey A. Head12David W. Greening13Cheng Xue Qin14Drug, Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash UniversityDrug, Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash UniversityBaker Heart & Diabetes InstituteDrug, Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash UniversityBaker Heart & Diabetes InstituteDrug, Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash UniversityDrug, Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash UniversityDrug, Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash UniversityBaker Heart & Diabetes InstituteMonash Biomedical Imaging, Monash University, ClaytonDrug, Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash UniversityDrug, Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash UniversityBaker Heart & Diabetes InstituteBaker Heart & Diabetes InstituteDrug, Discovery Biology, Monash Institute of Pharmaceutical Sciences, Monash UniversityAbstract Cardiovascular disease exhibits distinct sex-based differences, yet the mechanisms underlying these differences remain under-explored. The pro-resolving mediator annexin-A1 (ANXA1) is a pivotal regulator in inflammation resolution and tissue homeostasis, including within the cardiovascular system. However, the sex-specific differences in ANXA1 in blood pressure regulation have not been investigated. Here, we demonstrate that deficiency of ANXA1 exacerbates angiotensin II-induced adverse aortic and cardiac structural remodeling, mitochondrial proteome dysregulation, and impaired mitochondrial function in preclinical hypertensive models, exacerbated in females. Mechanistically, we demonstrate that estrogen upregulates ANXA1 levels, associated with dysregulation of inflammatory and mitochondrial networks, suggesting that the estrogen-ANXA1 axis plays a critical role in modulating inflammation and preventing pathological remodeling. In conclusion, this study advances the understanding of female-specific cardiac and aortic tissue and cellular alterations in hypertension, providing a platform for developing therapeutic ANXA1 mimetics that address the unique pathophysiological features of hypertension in females.https://doi.org/10.1038/s42003-025-08291-6
spellingShingle Jaideep Singh
Kristy L. Jackson
Haoyun Fang
Feng Shii Tang
Cindy Gueguen
Alex M. Parker
HsinErh Chen
Cameron J. Nowell
Helen Kiriazis
Ekaterina Salimova
Owen L. Woodman
Rebecca H. Ritchie
Geoffrey A. Head
David W. Greening
Cheng Xue Qin
Annexin-A1 deficiency uncovers female-specific pathways in blood pressure control and cardiovascular remodeling in mice
Communications Biology
title Annexin-A1 deficiency uncovers female-specific pathways in blood pressure control and cardiovascular remodeling in mice
title_full Annexin-A1 deficiency uncovers female-specific pathways in blood pressure control and cardiovascular remodeling in mice
title_fullStr Annexin-A1 deficiency uncovers female-specific pathways in blood pressure control and cardiovascular remodeling in mice
title_full_unstemmed Annexin-A1 deficiency uncovers female-specific pathways in blood pressure control and cardiovascular remodeling in mice
title_short Annexin-A1 deficiency uncovers female-specific pathways in blood pressure control and cardiovascular remodeling in mice
title_sort annexin a1 deficiency uncovers female specific pathways in blood pressure control and cardiovascular remodeling in mice
url https://doi.org/10.1038/s42003-025-08291-6
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