MiR-21-5p enhances differentiation and mitigates oleic acid-induced lipid droplet accumulation in C2C12 myoblasts by targeting FBXO11
Objective This study aimed to investigate the role of miRNA 21-5p in regulating the differentiation of C2C12 myoblasts and intramuscular lipid droplets accumulation in myotubes. Methods The role of miR-21-5p in the proliferation and differentiation of myofibroblasts and intracellular lipid accumulat...
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| Format: | Article |
| Language: | English |
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Asian-Australasian Association of Animal Production Societies
2025-06-01
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| Series: | Animal Bioscience |
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| Online Access: | http://www.animbiosci.org/upload/pdf/ab-24-0665.pdf |
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| author | Yi Li Jiayuan Fang Yingying Jiao Qinchuan Lv Xingyu Xiao Shuo Zheng Xi Chen Jie Song Xunming Zhang Libo Zhang Ze Ma Changhong Li Linlin Hao |
| author_facet | Yi Li Jiayuan Fang Yingying Jiao Qinchuan Lv Xingyu Xiao Shuo Zheng Xi Chen Jie Song Xunming Zhang Libo Zhang Ze Ma Changhong Li Linlin Hao |
| author_sort | Yi Li |
| collection | DOAJ |
| description | Objective This study aimed to investigate the role of miRNA 21-5p in regulating the differentiation of C2C12 myoblasts and intramuscular lipid droplets accumulation in myotubes. Methods The role of miR-21-5p in the proliferation and differentiation of myofibroblasts and intracellular lipid accumulation was analyzed using bioinformatics, CCK-8 assay, quantitative real-time polymerase chain reaction, immunoblotting, immunofluorescence staining, and Oil Red O staining. Results The analysis of porcine BodyMap transcriptome data revealed differential expression of miRNA 21-5p in skeletal muscle and adipose tissue. Bioinformatics analysis combined with a dual-luciferase reporter assay demonstrated that FBXO11 serves as a direct target of miR-21-5p. Transfection experiments involving a miR-21-5p mimic, miR-21-5p inhibitor, and si-FBXO11 in C2C12 cells showed that overexpression of miR-21-5p or silencing of FBXO11 significantly enhanced the proliferation of C2C12 cells, upregulated myogenesis-related factors, and promoted myotube formation. Furthermore, oleic acid-induced lipid accumulation in myotubes was suppressed, accompanied by reduced expression of adipogenesis-related genes. Conversely, inhibition of miR-21-5p expression produced opposite effects. Conclusion These findings indicate that miR-21-5p promotes proliferation and differentiation while inhibiting intramyocellular lipid deposition by targeting the 3′-untranslated region of FBXO11 in myogenic cell. The results suggest that miR-21-5p could serve as a potential miRNA biomarker for regulating intramuscular adipogenesis. |
| format | Article |
| id | doaj-art-7ba44ea25608474089de3e449b125358 |
| institution | OA Journals |
| issn | 2765-0189 2765-0235 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Asian-Australasian Association of Animal Production Societies |
| record_format | Article |
| series | Animal Bioscience |
| spelling | doaj-art-7ba44ea25608474089de3e449b1253582025-08-20T02:28:19ZengAsian-Australasian Association of Animal Production SocietiesAnimal Bioscience2765-01892765-02352025-06-013861279129010.5713/ab.24.066525431MiR-21-5p enhances differentiation and mitigates oleic acid-induced lipid droplet accumulation in C2C12 myoblasts by targeting FBXO11Yi Li0Jiayuan Fang1Yingying Jiao2Qinchuan Lv3Xingyu Xiao4Shuo Zheng5Xi Chen6Jie Song7Xunming Zhang8Libo Zhang9Ze Ma10Changhong Li11Linlin Hao12 College of Animal Science, Jilin University, Changchun, China College of Animal Science, Jilin University, Changchun, China College of Animal Science, Jilin University, Changchun, China College of Animal Science, Jilin University, Changchun, China College of Animal Science, Jilin University, Changchun, China College of Animal Science, Jilin University, Changchun, China College of Animal Science, Jilin University, Changchun, China College of Animal Science, Jilin University, Changchun, China College of Animal Science, Jilin University, Changchun, China College of Animal Science, Jilin University, Changchun, China College of Animal Science, Jilin University, Changchun, China College of Life Science, Baicheng Normal University, Baicheng, China College of Animal Science, Jilin University, Changchun, ChinaObjective This study aimed to investigate the role of miRNA 21-5p in regulating the differentiation of C2C12 myoblasts and intramuscular lipid droplets accumulation in myotubes. Methods The role of miR-21-5p in the proliferation and differentiation of myofibroblasts and intracellular lipid accumulation was analyzed using bioinformatics, CCK-8 assay, quantitative real-time polymerase chain reaction, immunoblotting, immunofluorescence staining, and Oil Red O staining. Results The analysis of porcine BodyMap transcriptome data revealed differential expression of miRNA 21-5p in skeletal muscle and adipose tissue. Bioinformatics analysis combined with a dual-luciferase reporter assay demonstrated that FBXO11 serves as a direct target of miR-21-5p. Transfection experiments involving a miR-21-5p mimic, miR-21-5p inhibitor, and si-FBXO11 in C2C12 cells showed that overexpression of miR-21-5p or silencing of FBXO11 significantly enhanced the proliferation of C2C12 cells, upregulated myogenesis-related factors, and promoted myotube formation. Furthermore, oleic acid-induced lipid accumulation in myotubes was suppressed, accompanied by reduced expression of adipogenesis-related genes. Conversely, inhibition of miR-21-5p expression produced opposite effects. Conclusion These findings indicate that miR-21-5p promotes proliferation and differentiation while inhibiting intramyocellular lipid deposition by targeting the 3′-untranslated region of FBXO11 in myogenic cell. The results suggest that miR-21-5p could serve as a potential miRNA biomarker for regulating intramuscular adipogenesis.http://www.animbiosci.org/upload/pdf/ab-24-0665.pdfc2c12 myoblastdifferentiationfbxo11intramuscular lipid depositionmir-21-5pproliferation |
| spellingShingle | Yi Li Jiayuan Fang Yingying Jiao Qinchuan Lv Xingyu Xiao Shuo Zheng Xi Chen Jie Song Xunming Zhang Libo Zhang Ze Ma Changhong Li Linlin Hao MiR-21-5p enhances differentiation and mitigates oleic acid-induced lipid droplet accumulation in C2C12 myoblasts by targeting FBXO11 Animal Bioscience c2c12 myoblast differentiation fbxo11 intramuscular lipid deposition mir-21-5p proliferation |
| title | MiR-21-5p enhances differentiation and mitigates oleic acid-induced lipid droplet accumulation in C2C12 myoblasts by targeting FBXO11 |
| title_full | MiR-21-5p enhances differentiation and mitigates oleic acid-induced lipid droplet accumulation in C2C12 myoblasts by targeting FBXO11 |
| title_fullStr | MiR-21-5p enhances differentiation and mitigates oleic acid-induced lipid droplet accumulation in C2C12 myoblasts by targeting FBXO11 |
| title_full_unstemmed | MiR-21-5p enhances differentiation and mitigates oleic acid-induced lipid droplet accumulation in C2C12 myoblasts by targeting FBXO11 |
| title_short | MiR-21-5p enhances differentiation and mitigates oleic acid-induced lipid droplet accumulation in C2C12 myoblasts by targeting FBXO11 |
| title_sort | mir 21 5p enhances differentiation and mitigates oleic acid induced lipid droplet accumulation in c2c12 myoblasts by targeting fbxo11 |
| topic | c2c12 myoblast differentiation fbxo11 intramuscular lipid deposition mir-21-5p proliferation |
| url | http://www.animbiosci.org/upload/pdf/ab-24-0665.pdf |
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