Substrate profiling of marine-derived thermotolerant cysteine protease reveals unique cleavage preferences for industrial applications

Substrate profiling of marine-derived thermotolerant cysteine protease reveals unique cleavage preferences for industrial applications

Abstract Due to their industrial importance, new proteases are constantly being sourced from the marine environment. However, their substrate specificities remain insufficiently studied, restricting the evaluation of their potential applications. Here, we applied multiplex substrate profiling by mas...

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Main Authors: Victoria Røyseth, Brianna M. Hurysz, Hasan Arsın, Julia M. Vazquez, Anna-Karina Kaczorowska, Anita-Elin Fedøy, Daria Biernacka, Sebastian Dorawa, Tadeusz Kaczorowski, Runar Stokke, Anthony J. O’Donoghue, Ida Helene Steen
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
Subjects:
Marine cysteine protease
Globupain
Clostripain
Multiplex substrate profiling by mass spectrometry
Marine bioprospecting
Hydrothermal vents
Online Access:https://doi.org/10.1038/s41598-025-11635-1
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https://doi.org/10.1038/s41598-025-11635-1

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