Quantification of Folded and Misfolded Proinsulin Forms Using Nonreducing SDS-PAGE and Proinsulin-Specific Immunoblotting

We have observed that some proinsulin molecules in pancreatic islets and beta cell lines have incomplete or improper intramolecular disulfide bonds. These misfolded monomers can form intermolecular disulfide-linked complexes. Accurately quantifying the fraction of proinsulin molecules contained in t...

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Bibliographic Details
Main Authors: Anoop Arunagiri, Insook Jang, Pamela Itkin-Ansari, Randal Kaufman, Peter Arvan
Format: Article
Language:English
Published: Bio-protocol LLC 2025-06-01
Series:Bio-Protocol
Online Access:https://bio-protocol.org/en/bpdetail?id=5337&type=0
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