Phenolic profiles and antioxidant activity of Morus alba L. infusions prepared from commercially available products and naturally collected leaves
Abstract The aim of this study was to compare the phenolic compound content and antioxidant activity of infusions made from commercially available Morus alba L. leaves with those of infusions made from a naturally collected source. The phenolic profile was determined using RP-HPLC–DAD and LC-Q-TOF–M...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-04-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-97223-9 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849694339779788800 |
|---|---|
| author | Michał Adam Janiak Anna Gryn-Rynko Katarzyna Sulewska Ryszard Amarowicz Kamila Penkacik Radomir Graczyk Dorota Olszewska-Słonina Michał Stanisław Majewski |
| author_facet | Michał Adam Janiak Anna Gryn-Rynko Katarzyna Sulewska Ryszard Amarowicz Kamila Penkacik Radomir Graczyk Dorota Olszewska-Słonina Michał Stanisław Majewski |
| author_sort | Michał Adam Janiak |
| collection | DOAJ |
| description | Abstract The aim of this study was to compare the phenolic compound content and antioxidant activity of infusions made from commercially available Morus alba L. leaves with those of infusions made from a naturally collected source. The phenolic profile was determined using RP-HPLC–DAD and LC-Q-TOF–MS/MS. The total phenolic content (TPC) was determined using Folin-Ciocâlteu reagent. To assess the antioxidant and reducing properties, Trolox equivalent antioxidant capacity (TEAC) and ferric reducing antioxidant power (FRAP) were used. Our analysis revealed the presence of two phenolic acids and six flavonoids. The most abundant compound was chlorogenic acid. The TPC, TEAC, and FRAP results indicated that infusions prepared from naturally collected samples exhibited greater phenolic content (19.7—52.6 vs 18.1—35.2 mg/100 ml) and stronger antioxidant (0.0605—0.1842 vs 0.0453—0.0822 mmol Trolox/100 ml) and reducing (0.244—0.597 vs 0.202—0.371 mmol Fe2+/100 ml) activities than those of commercially available products in the Polish market. Samples L1-L3 from the natural collection were those with the highest values. These results were further supported by principal component analysis (PCA), which categorized observations into three distinct clusters. |
| format | Article |
| id | doaj-art-7abc6a38d3b1402ca12660c6f8e46753 |
| institution | DOAJ |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-7abc6a38d3b1402ca12660c6f8e467532025-08-20T03:20:06ZengNature PortfolioScientific Reports2045-23222025-04-0115111210.1038/s41598-025-97223-9Phenolic profiles and antioxidant activity of Morus alba L. infusions prepared from commercially available products and naturally collected leavesMichał Adam Janiak0Anna Gryn-Rynko1Katarzyna Sulewska2Ryszard Amarowicz3Kamila Penkacik4Radomir Graczyk5Dorota Olszewska-Słonina6Michał Stanisław Majewski7Scientific Group of Chemical and Physical Properties of Food, Institute of Animal Reproduction and Food Research of Polish Academy of SciencesDepartment of Pharmacology and Toxicology, Faculty of Medicine, University of Warmia and Mazury in OlsztynScientific Group of Chemical and Physical Properties of Food, Institute of Animal Reproduction and Food Research of Polish Academy of SciencesScientific Group of Chemical and Physical Properties of Food, Institute of Animal Reproduction and Food Research of Polish Academy of SciencesScientific Group of Chemical and Physical Properties of Food, Institute of Animal Reproduction and Food Research of Polish Academy of SciencesDepartment of Biology and Animal Environment, Faculty of Animal Breeding and Biology, Bydgoszcz University of Science and TechnologyDepartment of Pathobiochemistry and Clinical Chemistry, Faculty of Pharmacy, Ludwik Rydygier Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in ToruńDepartment of Pharmacology and Toxicology, Faculty of Medicine, University of Warmia and Mazury in OlsztynAbstract The aim of this study was to compare the phenolic compound content and antioxidant activity of infusions made from commercially available Morus alba L. leaves with those of infusions made from a naturally collected source. The phenolic profile was determined using RP-HPLC–DAD and LC-Q-TOF–MS/MS. The total phenolic content (TPC) was determined using Folin-Ciocâlteu reagent. To assess the antioxidant and reducing properties, Trolox equivalent antioxidant capacity (TEAC) and ferric reducing antioxidant power (FRAP) were used. Our analysis revealed the presence of two phenolic acids and six flavonoids. The most abundant compound was chlorogenic acid. The TPC, TEAC, and FRAP results indicated that infusions prepared from naturally collected samples exhibited greater phenolic content (19.7—52.6 vs 18.1—35.2 mg/100 ml) and stronger antioxidant (0.0605—0.1842 vs 0.0453—0.0822 mmol Trolox/100 ml) and reducing (0.244—0.597 vs 0.202—0.371 mmol Fe2+/100 ml) activities than those of commercially available products in the Polish market. Samples L1-L3 from the natural collection were those with the highest values. These results were further supported by principal component analysis (PCA), which categorized observations into three distinct clusters.https://doi.org/10.1038/s41598-025-97223-9Morus alba L.Phenolic compoundsAntioxidant activityRP-HPLC-DADChlorogenic acidLC-Q-TOF-MS/MS |
| spellingShingle | Michał Adam Janiak Anna Gryn-Rynko Katarzyna Sulewska Ryszard Amarowicz Kamila Penkacik Radomir Graczyk Dorota Olszewska-Słonina Michał Stanisław Majewski Phenolic profiles and antioxidant activity of Morus alba L. infusions prepared from commercially available products and naturally collected leaves Scientific Reports Morus alba L. Phenolic compounds Antioxidant activity RP-HPLC-DAD Chlorogenic acid LC-Q-TOF-MS/MS |
| title | Phenolic profiles and antioxidant activity of Morus alba L. infusions prepared from commercially available products and naturally collected leaves |
| title_full | Phenolic profiles and antioxidant activity of Morus alba L. infusions prepared from commercially available products and naturally collected leaves |
| title_fullStr | Phenolic profiles and antioxidant activity of Morus alba L. infusions prepared from commercially available products and naturally collected leaves |
| title_full_unstemmed | Phenolic profiles and antioxidant activity of Morus alba L. infusions prepared from commercially available products and naturally collected leaves |
| title_short | Phenolic profiles and antioxidant activity of Morus alba L. infusions prepared from commercially available products and naturally collected leaves |
| title_sort | phenolic profiles and antioxidant activity of morus alba l infusions prepared from commercially available products and naturally collected leaves |
| topic | Morus alba L. Phenolic compounds Antioxidant activity RP-HPLC-DAD Chlorogenic acid LC-Q-TOF-MS/MS |
| url | https://doi.org/10.1038/s41598-025-97223-9 |
| work_keys_str_mv | AT michaładamjaniak phenolicprofilesandantioxidantactivityofmorusalbalinfusionspreparedfromcommerciallyavailableproductsandnaturallycollectedleaves AT annagrynrynko phenolicprofilesandantioxidantactivityofmorusalbalinfusionspreparedfromcommerciallyavailableproductsandnaturallycollectedleaves AT katarzynasulewska phenolicprofilesandantioxidantactivityofmorusalbalinfusionspreparedfromcommerciallyavailableproductsandnaturallycollectedleaves AT ryszardamarowicz phenolicprofilesandantioxidantactivityofmorusalbalinfusionspreparedfromcommerciallyavailableproductsandnaturallycollectedleaves AT kamilapenkacik phenolicprofilesandantioxidantactivityofmorusalbalinfusionspreparedfromcommerciallyavailableproductsandnaturallycollectedleaves AT radomirgraczyk phenolicprofilesandantioxidantactivityofmorusalbalinfusionspreparedfromcommerciallyavailableproductsandnaturallycollectedleaves AT dorotaolszewskasłonina phenolicprofilesandantioxidantactivityofmorusalbalinfusionspreparedfromcommerciallyavailableproductsandnaturallycollectedleaves AT michałstanisławmajewski phenolicprofilesandantioxidantactivityofmorusalbalinfusionspreparedfromcommerciallyavailableproductsandnaturallycollectedleaves |