Protocol for isolating the single bacteriocyte from whiteflies for single-cell RNA-seq analysis
Summary: Bacteriocytes are specialized insect cells adapted to harbor symbionts. However, their low number in individual whiteflies makes obtaining enough for transcriptome sequencing challenging. Here, we present a protocol for the isolation of whitefly bacteriocytes. We describe steps for preparin...
Saved in:
| Main Authors: | Na-Na Li, Hui Quan, Jun-Bo Luan |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000978 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Protocol update to: High-throughput scNMT protocol for multiomics profiling of single cells from mouse brain and pancreatic organoids
by: Santiago Cerrizuela, et al.
Published: (2025-09-01) -
Protocol for interpretable and context-specific single-cell-informed deconvolution of bulk RNA-seq data
by: Daniele Malpetti, et al.
Published: (2025-03-01) -
Protocol for high-quality RNA sequencing, cell surface protein analysis, and genotyping in single cells using TARGET-seq+
by: N. Asger Jakobsen, et al.
Published: (2025-06-01) -
Characterization of Loss-of-Imprinting in Breast Cancer at the Cellular Level by Integrating Single-Cell Full-Length Transcriptome with Bulk RNA-Seq Data
by: Muhammad Talal Amin, et al.
Published: (2024-12-01) -
Advances in Microfluidic Single-Cell RNA Sequencing and Spatial Transcriptomics
by: Yueqiu Sun, et al.
Published: (2025-04-01)