Protocol for isolating the single bacteriocyte from whiteflies for single-cell RNA-seq analysis

Summary: Bacteriocytes are specialized insect cells adapted to harbor symbionts. However, their low number in individual whiteflies makes obtaining enough for transcriptome sequencing challenging. Here, we present a protocol for the isolation of whitefly bacteriocytes. We describe steps for preparin...

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Bibliographic Details
Main Authors: Na-Na Li, Hui Quan, Jun-Bo Luan
Format: Article
Language:English
Published: Elsevier 2025-03-01
Series:STAR Protocols
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725000978
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