Protocol for isolating the single bacteriocyte from whiteflies for single-cell RNA-seq analysis
Summary: Bacteriocytes are specialized insect cells adapted to harbor symbionts. However, their low number in individual whiteflies makes obtaining enough for transcriptome sequencing challenging. Here, we present a protocol for the isolation of whitefly bacteriocytes. We describe steps for preparin...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000978 |
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| author | Na-Na Li Hui Quan Jun-Bo Luan |
| author_facet | Na-Na Li Hui Quan Jun-Bo Luan |
| author_sort | Na-Na Li |
| collection | DOAJ |
| description | Summary: Bacteriocytes are specialized insect cells adapted to harbor symbionts. However, their low number in individual whiteflies makes obtaining enough for transcriptome sequencing challenging. Here, we present a protocol for the isolation of whitefly bacteriocytes. We describe steps for preparing the single bacteriocyte sample, cDNA amplification library construction, sequencing, and data analysis. Our protocol offers convenience and efficiency for conducting single-cell sequencing for bacteriocytes and has the potential to be applied to other insect cells.For complete details on the use and execution of this protocol, please refer to Li et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-7a29fd161dff41b8aa89693ce4e927eb |
| institution | OA Journals |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-7a29fd161dff41b8aa89693ce4e927eb2025-08-20T02:05:12ZengElsevierSTAR Protocols2666-16672025-03-016110369110.1016/j.xpro.2025.103691Protocol for isolating the single bacteriocyte from whiteflies for single-cell RNA-seq analysisNa-Na Li0Hui Quan1Jun-Bo Luan2Liaoning Key Laboratory of Economic and Applied Entomology, College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, ChinaAnnoroad Gene Technology Co., Ltd., Beijing 100176, ChinaLiaoning Key Laboratory of Economic and Applied Entomology, College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China; Shenyang Key Laboratory of Surveillance and Management for Vegetable Diseases and Insect Pests, College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China; Corresponding authorSummary: Bacteriocytes are specialized insect cells adapted to harbor symbionts. However, their low number in individual whiteflies makes obtaining enough for transcriptome sequencing challenging. Here, we present a protocol for the isolation of whitefly bacteriocytes. We describe steps for preparing the single bacteriocyte sample, cDNA amplification library construction, sequencing, and data analysis. Our protocol offers convenience and efficiency for conducting single-cell sequencing for bacteriocytes and has the potential to be applied to other insect cells.For complete details on the use and execution of this protocol, please refer to Li et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000978Cell BiologySingle CellGenomicsSequencingRNA-seqModel Organisms |
| spellingShingle | Na-Na Li Hui Quan Jun-Bo Luan Protocol for isolating the single bacteriocyte from whiteflies for single-cell RNA-seq analysis STAR Protocols Cell Biology Single Cell Genomics Sequencing RNA-seq Model Organisms |
| title | Protocol for isolating the single bacteriocyte from whiteflies for single-cell RNA-seq analysis |
| title_full | Protocol for isolating the single bacteriocyte from whiteflies for single-cell RNA-seq analysis |
| title_fullStr | Protocol for isolating the single bacteriocyte from whiteflies for single-cell RNA-seq analysis |
| title_full_unstemmed | Protocol for isolating the single bacteriocyte from whiteflies for single-cell RNA-seq analysis |
| title_short | Protocol for isolating the single bacteriocyte from whiteflies for single-cell RNA-seq analysis |
| title_sort | protocol for isolating the single bacteriocyte from whiteflies for single cell rna seq analysis |
| topic | Cell Biology Single Cell Genomics Sequencing RNA-seq Model Organisms |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000978 |
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