Excessive exercise elicits poly (ADP-ribose) Polymerase-1 activation and global protein PARylation driving muscle dysfunction and performance impairment

Excessive exercise combined with inadequate recovery time may trigger fatigue, performance impairment, and ultimately the overtraining syndrome. The intramyocellular mechanisms involved in the overtraining syndrome remain only partially known. Here, we combined multi-omics analyses from isogenic BXD...

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Main Authors: Barbara M. Crisol, Matheus B. Rocha, Beatriz Franco, Ana Paula Morelli, Carlos K. Katashima, Scylas J.A. Junior, Fernanda S. Carneiro, Renata R. Braga, Rafael S. Brícola, Graciana de Azambuja, Raul Gobato Costa, Andrea M. Esteves, Marcelo A. Mori, Maria C.G. Oliveira, Dennys E. Cintra, José R. Pauli, Filip J. Larsen, Adelino S.R. da Silva, Eduardo R. Ropelle
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Molecular Metabolism
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Online Access:http://www.sciencedirect.com/science/article/pii/S2212877825000420
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author Barbara M. Crisol
Matheus B. Rocha
Beatriz Franco
Ana Paula Morelli
Carlos K. Katashima
Scylas J.A. Junior
Fernanda S. Carneiro
Renata R. Braga
Rafael S. Brícola
Graciana de Azambuja
Raul Gobato Costa
Andrea M. Esteves
Marcelo A. Mori
Maria C.G. Oliveira
Dennys E. Cintra
José R. Pauli
Filip J. Larsen
Adelino S.R. da Silva
Eduardo R. Ropelle
author_facet Barbara M. Crisol
Matheus B. Rocha
Beatriz Franco
Ana Paula Morelli
Carlos K. Katashima
Scylas J.A. Junior
Fernanda S. Carneiro
Renata R. Braga
Rafael S. Brícola
Graciana de Azambuja
Raul Gobato Costa
Andrea M. Esteves
Marcelo A. Mori
Maria C.G. Oliveira
Dennys E. Cintra
José R. Pauli
Filip J. Larsen
Adelino S.R. da Silva
Eduardo R. Ropelle
author_sort Barbara M. Crisol
collection DOAJ
description Excessive exercise combined with inadequate recovery time may trigger fatigue, performance impairment, and ultimately the overtraining syndrome. The intramyocellular mechanisms involved in the overtraining syndrome remain only partially known. Here, we combined multi-omics analyses from isogenic BXD mouse strains with a mouse model of overtraining and excessive exercise protocol in mice and humans to evaluate the molecular mechanism involved in the performance impairment induced by excessive exercise. We identified that BXD mouse strains with elevated levels of Parp1 gene expression in the skeletal muscle displayed features like overtraining syndrome and abnormal muscle genetic signature. High PARP1 protein content and aberrant PARylation of proteins were detected in the skeletal muscle of overtrained, but not in trained mice. Overtraining syndrome reduced mitochondrial function promoted by exercise training, induced muscle hyperalgesia, reduced muscle fiber size and promoted a similar gene signature of myopathy and atrophy models. Short periods of excessive exercise also increased PARylation in the skeletal muscle of mice and healthy subjects. The pharmacological inhibition of PARP1, using Olaparib, and genetic Parp1 ablation, preserved muscle fiber morphology and protected against physical performance impairment and other symptoms of the overtraining syndrome in mice. In conclusion, PARP1 excessive activation is related to muscle abnormalities led by long or short periods of excessive exercise, and here we suggest that PARP1 is a potential target in the treatment and prevention of overtraining syndrome.
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spelling doaj-art-79bd0ccb904d4e399ab423704dadbc7b2025-08-20T03:48:50ZengElsevierMolecular Metabolism2212-87782025-06-019610213510.1016/j.molmet.2025.102135Excessive exercise elicits poly (ADP-ribose) Polymerase-1 activation and global protein PARylation driving muscle dysfunction and performance impairmentBarbara M. Crisol0Matheus B. Rocha1Beatriz Franco2Ana Paula Morelli3Carlos K. Katashima4Scylas J.A. Junior5Fernanda S. Carneiro6Renata R. Braga7Rafael S. Brícola8Graciana de Azambuja9Raul Gobato Costa10Andrea M. Esteves11Marcelo A. Mori12Maria C.G. Oliveira13Dennys E. Cintra14José R. Pauli15Filip J. Larsen16Adelino S.R. da Silva17Eduardo R. Ropelle18Laboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP, 13484-350, BrazilLaboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP, 13484-350, BrazilSchool of Applied Sciences, University of Campinas, Limeira, SP, BrazilLaboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP, 13484-350, BrazilLaboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP, 13484-350, BrazilLaboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP, 13484-350, BrazilLaboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP, 13484-350, BrazilLaboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP, 13484-350, BrazilLaboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP, 13484-350, BrazilSchool of Applied Sciences, University of Campinas, Limeira, SP, BrazilDepartment of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), BrazilSchool of Applied Sciences, University of Campinas, Limeira, SP, BrazilDepartment of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), BrazilSchool of Applied Sciences, University of Campinas, Limeira, SP, BrazilLaboratory of Nutritional Genomic, School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP, 13484-350, Brazil; Obesity and Comorbidities Research Center, University of Campinas (UNICAMP), BrazilLaboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP, 13484-350, Brazil; School of Applied Sciences, University of Campinas, Limeira, SP, Brazil; Obesity and Comorbidities Research Center, University of Campinas (UNICAMP), BrazilThe Swedish School of Sport and Health Sciences, GIH, Åstrand Laboratory, Department of Physiology, Nutrition and Biomechanics, Stockholm, 114 33, SwedenPostgraduate Program in Rehabilitation and Functional Performance, Ribeirão Preto Medical School, University of São Paulo (USP), Ribeirão Preto, SP, Brazil; School of Physical Education and Sport of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, BrazilLaboratory of Molecular Biology of Exercise (LaBMEx), School of Applied Sciences, University of Campinas (UNICAMP), Limeira, SP, 13484-350, Brazil; School of Applied Sciences, University of Campinas, Limeira, SP, Brazil; Obesity and Comorbidities Research Center, University of Campinas (UNICAMP), Brazil; Corresponding author. University of Campinas Laboratory of Molecular Biology of Exercise, 13484-350, Limeira, SP, Brazil.Excessive exercise combined with inadequate recovery time may trigger fatigue, performance impairment, and ultimately the overtraining syndrome. The intramyocellular mechanisms involved in the overtraining syndrome remain only partially known. Here, we combined multi-omics analyses from isogenic BXD mouse strains with a mouse model of overtraining and excessive exercise protocol in mice and humans to evaluate the molecular mechanism involved in the performance impairment induced by excessive exercise. We identified that BXD mouse strains with elevated levels of Parp1 gene expression in the skeletal muscle displayed features like overtraining syndrome and abnormal muscle genetic signature. High PARP1 protein content and aberrant PARylation of proteins were detected in the skeletal muscle of overtrained, but not in trained mice. Overtraining syndrome reduced mitochondrial function promoted by exercise training, induced muscle hyperalgesia, reduced muscle fiber size and promoted a similar gene signature of myopathy and atrophy models. Short periods of excessive exercise also increased PARylation in the skeletal muscle of mice and healthy subjects. The pharmacological inhibition of PARP1, using Olaparib, and genetic Parp1 ablation, preserved muscle fiber morphology and protected against physical performance impairment and other symptoms of the overtraining syndrome in mice. In conclusion, PARP1 excessive activation is related to muscle abnormalities led by long or short periods of excessive exercise, and here we suggest that PARP1 is a potential target in the treatment and prevention of overtraining syndrome.http://www.sciencedirect.com/science/article/pii/S2212877825000420ExerciseMusclePARP1overtraining
spellingShingle Barbara M. Crisol
Matheus B. Rocha
Beatriz Franco
Ana Paula Morelli
Carlos K. Katashima
Scylas J.A. Junior
Fernanda S. Carneiro
Renata R. Braga
Rafael S. Brícola
Graciana de Azambuja
Raul Gobato Costa
Andrea M. Esteves
Marcelo A. Mori
Maria C.G. Oliveira
Dennys E. Cintra
José R. Pauli
Filip J. Larsen
Adelino S.R. da Silva
Eduardo R. Ropelle
Excessive exercise elicits poly (ADP-ribose) Polymerase-1 activation and global protein PARylation driving muscle dysfunction and performance impairment
Molecular Metabolism
Exercise
Muscle
PARP1
overtraining
title Excessive exercise elicits poly (ADP-ribose) Polymerase-1 activation and global protein PARylation driving muscle dysfunction and performance impairment
title_full Excessive exercise elicits poly (ADP-ribose) Polymerase-1 activation and global protein PARylation driving muscle dysfunction and performance impairment
title_fullStr Excessive exercise elicits poly (ADP-ribose) Polymerase-1 activation and global protein PARylation driving muscle dysfunction and performance impairment
title_full_unstemmed Excessive exercise elicits poly (ADP-ribose) Polymerase-1 activation and global protein PARylation driving muscle dysfunction and performance impairment
title_short Excessive exercise elicits poly (ADP-ribose) Polymerase-1 activation and global protein PARylation driving muscle dysfunction and performance impairment
title_sort excessive exercise elicits poly adp ribose polymerase 1 activation and global protein parylation driving muscle dysfunction and performance impairment
topic Exercise
Muscle
PARP1
overtraining
url http://www.sciencedirect.com/science/article/pii/S2212877825000420
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