Biomineralization Performance of Bacillus sphaericus under the Action of Bacillus mucilaginosus
Microbial Induced Calcite Precipitation (MICP) is a biochemical process widely found in nature, also known as microbial mineralization. This paper investigates whether this process can help promote the intelligent reinforcement and repair of underground projects such as mines and tunnels. We selecte...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Advances in Materials Science and Engineering |
| Online Access: | http://dx.doi.org/10.1155/2020/6483803 |
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| author | Yingying Hu Weitao Liu Wenjing Wang Xinlei Jia Lanjuan Xu Qing Cao Jianjun Shen Xiangming Hu |
| author_facet | Yingying Hu Weitao Liu Wenjing Wang Xinlei Jia Lanjuan Xu Qing Cao Jianjun Shen Xiangming Hu |
| author_sort | Yingying Hu |
| collection | DOAJ |
| description | Microbial Induced Calcite Precipitation (MICP) is a biochemical process widely found in nature, also known as microbial mineralization. This paper investigates whether this process can help promote the intelligent reinforcement and repair of underground projects such as mines and tunnels. We selected Bacillus sphaericus and Bacillus mucilaginosus as the research objects. The former has an outstanding urease production ability, and the latter can secrete carbonic anhydrase in vitro. Bacillus mucilaginosus was introduced into the culture solution of Bacillus sphaericus in the most suitable culture environment, and the changes of mineralization rate and mineralization yield of Bacillus sphaericus were observed and analyzed. The results revealed that, to maintain the highest growth rate of Bacillus sphaericus, the optimal pH value was between 7 and 8, the optimal urea concentration was 0.5 mol/L, the optimal Ca2+ concentration was 0.6 mol/L, and the optimal Luria-Bertani (LB) culture concentration was 20 g/L. The amount of biomineralized calcium carbonate precipitated in the double bacteria solution can reach 1.89 times the amount of the precipitation in the Bacillus sphaericus solution under the same conditions. It concludes that the introduction of Bacillus mucilaginosus can effectively increase the mineralization yield of Bacillus sphaericus without affecting the mineralized products. |
| format | Article |
| id | doaj-art-787129a10cad4c9ba074d7ca9226685a |
| institution | Kabale University |
| issn | 1687-8434 1687-8442 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Materials Science and Engineering |
| spelling | doaj-art-787129a10cad4c9ba074d7ca9226685a2025-02-03T01:27:55ZengWileyAdvances in Materials Science and Engineering1687-84341687-84422020-01-01202010.1155/2020/64838036483803Biomineralization Performance of Bacillus sphaericus under the Action of Bacillus mucilaginosusYingying Hu0Weitao Liu1Wenjing Wang2Xinlei Jia3Lanjuan Xu4Qing Cao5Jianjun Shen6Xiangming Hu7College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266590, ChinaCollege of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266590, ChinaDepartment of Mechanical Engineering, University of Alberta, Edmonton, Alberta, CanadaCollege of Chemical Engineering and Safety, Bin Zhou University, Binzhou 256600, ChinaCollege of Chemical Engineering and Safety, Bin Zhou University, Binzhou 256600, ChinaCollege of Chemical Engineering and Safety, Bin Zhou University, Binzhou 256600, ChinaCollege of Chemical Engineering and Safety, Bin Zhou University, Binzhou 256600, ChinaCollege of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266590, ChinaMicrobial Induced Calcite Precipitation (MICP) is a biochemical process widely found in nature, also known as microbial mineralization. This paper investigates whether this process can help promote the intelligent reinforcement and repair of underground projects such as mines and tunnels. We selected Bacillus sphaericus and Bacillus mucilaginosus as the research objects. The former has an outstanding urease production ability, and the latter can secrete carbonic anhydrase in vitro. Bacillus mucilaginosus was introduced into the culture solution of Bacillus sphaericus in the most suitable culture environment, and the changes of mineralization rate and mineralization yield of Bacillus sphaericus were observed and analyzed. The results revealed that, to maintain the highest growth rate of Bacillus sphaericus, the optimal pH value was between 7 and 8, the optimal urea concentration was 0.5 mol/L, the optimal Ca2+ concentration was 0.6 mol/L, and the optimal Luria-Bertani (LB) culture concentration was 20 g/L. The amount of biomineralized calcium carbonate precipitated in the double bacteria solution can reach 1.89 times the amount of the precipitation in the Bacillus sphaericus solution under the same conditions. It concludes that the introduction of Bacillus mucilaginosus can effectively increase the mineralization yield of Bacillus sphaericus without affecting the mineralized products.http://dx.doi.org/10.1155/2020/6483803 |
| spellingShingle | Yingying Hu Weitao Liu Wenjing Wang Xinlei Jia Lanjuan Xu Qing Cao Jianjun Shen Xiangming Hu Biomineralization Performance of Bacillus sphaericus under the Action of Bacillus mucilaginosus Advances in Materials Science and Engineering |
| title | Biomineralization Performance of Bacillus sphaericus under the Action of Bacillus mucilaginosus |
| title_full | Biomineralization Performance of Bacillus sphaericus under the Action of Bacillus mucilaginosus |
| title_fullStr | Biomineralization Performance of Bacillus sphaericus under the Action of Bacillus mucilaginosus |
| title_full_unstemmed | Biomineralization Performance of Bacillus sphaericus under the Action of Bacillus mucilaginosus |
| title_short | Biomineralization Performance of Bacillus sphaericus under the Action of Bacillus mucilaginosus |
| title_sort | biomineralization performance of bacillus sphaericus under the action of bacillus mucilaginosus |
| url | http://dx.doi.org/10.1155/2020/6483803 |
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