Optimization and calibration of 384-well kinetic Ca2+ mobilization assays for the human transient receptor potential cation channels TRPM8, TRPV1, and TRPA1

Optimization and calibration of 384-well kinetic Ca2+ mobilization assays for the human transient receptor potential cation channels TRPM8, TRPV1, and TRPA1

Development, optimization, and calibration of human transient receptor potential (TRP) channel Ca2+ mobilization assays for TRPM8, TRPV1, and TRPA1 are described. Heterologous expression of hTRPM8 in HEK293T cells was required for anti-TRPM8 antibody staining and TRPM8 agonist induced Ca2+ mobilizat...

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Bibliographic Details
Main Authors: David A. Close, V. Blair Journigan, Paul A. Johnston
Format: Article
Language:English
Published: Elsevier 2025-01-01
Series:SLAS Discovery
Subjects:
Human transient receptor potential cation channels TRPM8
TRPV1
and TRPA1 Kinetic Ca2+ mobilization assays
Fold over baseline ratio data processing
Benchmarking with known agonists and antagonists
Online Access:http://www.sciencedirect.com/science/article/pii/S2472555224000698
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