Possible Involvement of Endothelin Peptides and L-Arginine-Nitric Oxide Pathway on the Effect of Endotoxin in the Rabbit Isolated Perfused Kidney
Escherichia coli endotoxin (LPS) when infused through the renal artery of the rabbit isolated perfused kidney prepared as constant pressure mode, caused a decrease in flow rate and kidney weight indicating its primary vasoconstrictor effect. This effect was predominant in kidneys from rabbits pretre...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
1994-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000293 |
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| Summary: | Escherichia coli endotoxin (LPS) when infused through the renal
artery of the rabbit isolated perfused kidney prepared as constant
pressure mode, caused a decrease in flow rate and kidney weight
indicating its primary vasoconstrictor effect. This effect was
predominant in kidneys from rabbits pretreated with LPS. Endothelin-1
at a concentration of 10−10 M and big endothelin-1 at a concentration
of 10−8 M produced equal vasoconstrictor effects in kidney. Addition
of endotheHn converting enzyme inhibitor, phosphoramidon, to the
perfusion medium at a concentration of 10−6 M caused a reduction in
the effects of both LPS and big ET-1 without altering the
vasoconstrictor effect of ETol. However, addition of methylene blue
(10−5 M), a soluble guanylate cyclase inhibitor and
NG-nitro-L-arginine-methyl ester (10−6 M) to the perfusion medium
caused a potentiation in the vasoconstrictor effect of LPS.
Indomethacin at a concentration of 10−6 M did not alter the effect of
LPS. These results were taken as evidence for the participation of
endothelin peptides and the L-arginine-nitric oxide pathway in the
effect ofLPS in rabbit isolated perfused kidney. |
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| ISSN: | 0962-9351 1466-1861 |