Microscopic and molecular detection of Cytauxzoon spp. in cats in Mosul city, Iraq
The study aims to diagnose Cytauxzoon spp. in affected stray cats in Mosul. To achieve this goal, microscopic and conventional polymerase chain reaction technology was used, and the genetic analysis of Cytauxzoon spp. was verified. Blood samples were collected from 50 stray cats in various city area...
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| Format: | Article |
| Language: | Arabic |
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University of Mosul, College of Veterinary Medicine
2025-01-01
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| Series: | Iraqi Journal of Veterinary Sciences |
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| Online Access: | https://www.vetmedmosul.com/article_186157_65e144e5b9157104edb0b29b2ef2ba16.pdf |
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| author | Sura S. Aghwan Enas S. Hussein Salam A Esmaeel |
| author_facet | Sura S. Aghwan Enas S. Hussein Salam A Esmaeel |
| author_sort | Sura S. Aghwan |
| collection | DOAJ |
| description | The study aims to diagnose Cytauxzoon spp. in affected stray cats in Mosul. To achieve this goal, microscopic and conventional polymerase chain reaction technology was used, and the genetic analysis of Cytauxzoon spp. was verified. Blood samples were collected from 50 stray cats in various city areas. The infection rate of Cytauxzoon spp. among cats in Mosul city was 76% (38/50). Using single sequence analysis, a single Cytauxzoon felis sequence was discovered in Nineveh Governorate for the first time. One of these sequences was submitted to GenBank with the entry number LC775709.1. The results indicate that some of the tested samples contain DNA from the Cytauxzoon genus, based on the presence of bands at the expected 1600 bp size. The local genome sequence of Cytauxzoon felis Sesa LC775709.1 is highly similar to sequences found in geographically diverse regions such as Switzerland, Italy, France, and the USA. According to the study, the infection rate of cats with Cytauxzoon spp parasites in Mosulwas significant, reaching 76%. The results of genetic sequencing were presented for the first time. One novel isolate was discovered: the Cytauxzoon felis Sesa gene LC775709.1 for 18S rRNA. The phylogenetic tree was constructed, and it was discovered that the local sequence LC775709.1 is very closely related genetically to the sequences from Europe and the USA while showing some genetic differences with the sequences from Brazil and South Africa. |
| format | Article |
| id | doaj-art-7782e84f615940a8bb1a6501dc5a2bfc |
| institution | Kabale University |
| issn | 1607-3894 2071-1255 |
| language | Arabic |
| publishDate | 2025-01-01 |
| publisher | University of Mosul, College of Veterinary Medicine |
| record_format | Article |
| series | Iraqi Journal of Veterinary Sciences |
| spelling | doaj-art-7782e84f615940a8bb1a6501dc5a2bfc2025-02-04T13:11:43ZaraUniversity of Mosul, College of Veterinary MedicineIraqi Journal of Veterinary Sciences1607-38942071-12552025-01-0139113514110.33899/ijvs.2024.151520.3759186157Microscopic and molecular detection of Cytauxzoon spp. in cats in Mosul city, IraqSura S. Aghwan0Enas S. Hussein1Salam A Esmaeel2Department of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Microbiology, College of Veterinary Medicine, University of Mosul, Mosul, IraqDepartment of Internal and Preventive Medicine, College of Veterinary Medicine, University of Mosul, Mosul, IraqThe study aims to diagnose Cytauxzoon spp. in affected stray cats in Mosul. To achieve this goal, microscopic and conventional polymerase chain reaction technology was used, and the genetic analysis of Cytauxzoon spp. was verified. Blood samples were collected from 50 stray cats in various city areas. The infection rate of Cytauxzoon spp. among cats in Mosul city was 76% (38/50). Using single sequence analysis, a single Cytauxzoon felis sequence was discovered in Nineveh Governorate for the first time. One of these sequences was submitted to GenBank with the entry number LC775709.1. The results indicate that some of the tested samples contain DNA from the Cytauxzoon genus, based on the presence of bands at the expected 1600 bp size. The local genome sequence of Cytauxzoon felis Sesa LC775709.1 is highly similar to sequences found in geographically diverse regions such as Switzerland, Italy, France, and the USA. According to the study, the infection rate of cats with Cytauxzoon spp parasites in Mosulwas significant, reaching 76%. The results of genetic sequencing were presented for the first time. One novel isolate was discovered: the Cytauxzoon felis Sesa gene LC775709.1 for 18S rRNA. The phylogenetic tree was constructed, and it was discovered that the local sequence LC775709.1 is very closely related genetically to the sequences from Europe and the USA while showing some genetic differences with the sequences from Brazil and South Africa.https://www.vetmedmosul.com/article_186157_65e144e5b9157104edb0b29b2ef2ba16.pdfcatsmolecularcytauxzoonosiscytauxzoon sppmosul |
| spellingShingle | Sura S. Aghwan Enas S. Hussein Salam A Esmaeel Microscopic and molecular detection of Cytauxzoon spp. in cats in Mosul city, Iraq Iraqi Journal of Veterinary Sciences cats molecular cytauxzoonosis cytauxzoon spp mosul |
| title | Microscopic and molecular detection of Cytauxzoon spp. in cats in Mosul city, Iraq |
| title_full | Microscopic and molecular detection of Cytauxzoon spp. in cats in Mosul city, Iraq |
| title_fullStr | Microscopic and molecular detection of Cytauxzoon spp. in cats in Mosul city, Iraq |
| title_full_unstemmed | Microscopic and molecular detection of Cytauxzoon spp. in cats in Mosul city, Iraq |
| title_short | Microscopic and molecular detection of Cytauxzoon spp. in cats in Mosul city, Iraq |
| title_sort | microscopic and molecular detection of cytauxzoon spp in cats in mosul city iraq |
| topic | cats molecular cytauxzoonosis cytauxzoon spp mosul |
| url | https://www.vetmedmosul.com/article_186157_65e144e5b9157104edb0b29b2ef2ba16.pdf |
| work_keys_str_mv | AT surasaghwan microscopicandmoleculardetectionofcytauxzoonsppincatsinmosulcityiraq AT enasshussein microscopicandmoleculardetectionofcytauxzoonsppincatsinmosulcityiraq AT salamaesmaeel microscopicandmoleculardetectionofcytauxzoonsppincatsinmosulcityiraq |