A sensitive sample preparation pipeline for adventitious virus detection using Oxford Nanopore sequencing

A sensitive sample preparation pipeline for adventitious virus detection using Oxford Nanopore sequencing

Recent regulatory guidance now encourages the use of sequencing as an alternative adventitious agent testing assay to lengthy compendial in vivo assays used for cell line qualification. Most short-read sequencing assays, however, still require over a week to obtain a final test result since the sequ...

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Bibliographic Details
Main Authors: Emmanuel K. Tsinda, Charles A. Swofford, James P.B. Strutt, José Sangerman, Raeuf Roushangar, Alexander Li, Lara M. Gomez, Aidan N. Bousquet, Caleb Neufeld, Jacqueline M. Wolfrum, Jongyoon Han, Rohan B.H. Williams, Anthony J. Sinskey, Paul W. Barone, Stacy L. Springs
Format: Article
Language:English
Published: Elsevier 2025-06-01
Series:Molecular Therapy: Methods & Clinical Development
Subjects:
cell therapy
long read sequencing
adventitious agent detection
biomanufacturing
SISPA
viral safety
Online Access:http://www.sciencedirect.com/science/article/pii/S2329050125000737
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