Chemical composition and glucose uptake effect on 3T3-L1 adipocytes of Ligustrum lucidum Ait. flowers
Ligustri lucidi Fructus is a traditional Chinese medicine and possesses various bioactivities, including hypoglycemic effect. Ligustrum lucidum Ait flowers are poorly investigated. Thus, we hypothesized that L. lucidum flowers also could have hypoglycemic effect. Chemical composition and glucose upt...
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Tsinghua University Press
2020-06-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453019302848 |
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| author | Lili Cui Jinmei Wang Mengke Wang Baoguang Wang Zhenhua Liu Jinfeng Wei Wenyi Kang |
| author_facet | Lili Cui Jinmei Wang Mengke Wang Baoguang Wang Zhenhua Liu Jinfeng Wei Wenyi Kang |
| author_sort | Lili Cui |
| collection | DOAJ |
| description | Ligustri lucidi Fructus is a traditional Chinese medicine and possesses various bioactivities, including hypoglycemic effect. Ligustrum lucidum Ait flowers are poorly investigated. Thus, we hypothesized that L. lucidum flowers also could have hypoglycemic effect. Chemical composition and glucose uptake effect of L. lucidum flowers on 3T3-L1 adipocytes were investigated. In this study, the components of L. lucidum flowers were investigated by various chromatographic and spectroscopic methods and the effects of L. lucidum flowers on the induction of glucose uptake were investigated by 3T3-L1 adipocytes. Seven compounds were isolated and identified from L. lucidum flowers, including ursolic acid (1), kaempferol-7-O-α-l-rhamnoside (2, KR), β-sitosterol (3), β-daucosterol (4), kaempferitrin (5, KF), 10-hydroxy oleuropein (6, HO), and kaempferol-3-O-β-d-glucopyranoside-7-α-l-rhamnopyranoside (7, KGR). The results of glucose uptake showed that total extract (TE), KR, KF and HO from L. lucidum flowers significantly enhanced glucose uptake. This study demonstrated that compounds 2, 5-7 were isolated for the first time from this plant and compounds 2 and 7 were isolated for the first time in genus Ligustrum, L. lucidum flowers, KR, KF and HO may possess potential hypoglycemic effect. |
| format | Article |
| id | doaj-art-76adbd8a95b04bd28bac4de8c6f586d2 |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2020-06-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-76adbd8a95b04bd28bac4de8c6f586d22025-02-02T23:24:54ZengTsinghua University PressFood Science and Human Wellness2213-45302020-06-0192124129Chemical composition and glucose uptake effect on 3T3-L1 adipocytes of Ligustrum lucidum Ait. flowersLili Cui0Jinmei Wang1Mengke Wang2Baoguang Wang3Zhenhua Liu4Jinfeng Wei5Wenyi Kang6National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, China; Kaifeng Key Laboratory of Functional Components in Health Food, Henan University, Kaifeng, 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Henan University, Kaifeng, 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, China; Joint International Research Laboratory of Food & Medicine Resource Function, Henan Province, Henan University, Kaifeng, 475004, ChinaNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, China; Kaifeng Key Laboratory of Functional Components in Health Food, Henan University, Kaifeng, 475004, China; Corresponding authors at: National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, China.National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, China; Kaifeng Key Laboratory of Functional Components in Health Food, Henan University, Kaifeng, 475004, China; Corresponding authors at: National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, China.National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, China; Kaifeng Key Laboratory of Functional Components in Health Food, Henan University, Kaifeng, 475004, China; Corresponding authors at: National R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng, 475004, China.Ligustri lucidi Fructus is a traditional Chinese medicine and possesses various bioactivities, including hypoglycemic effect. Ligustrum lucidum Ait flowers are poorly investigated. Thus, we hypothesized that L. lucidum flowers also could have hypoglycemic effect. Chemical composition and glucose uptake effect of L. lucidum flowers on 3T3-L1 adipocytes were investigated. In this study, the components of L. lucidum flowers were investigated by various chromatographic and spectroscopic methods and the effects of L. lucidum flowers on the induction of glucose uptake were investigated by 3T3-L1 adipocytes. Seven compounds were isolated and identified from L. lucidum flowers, including ursolic acid (1), kaempferol-7-O-α-l-rhamnoside (2, KR), β-sitosterol (3), β-daucosterol (4), kaempferitrin (5, KF), 10-hydroxy oleuropein (6, HO), and kaempferol-3-O-β-d-glucopyranoside-7-α-l-rhamnopyranoside (7, KGR). The results of glucose uptake showed that total extract (TE), KR, KF and HO from L. lucidum flowers significantly enhanced glucose uptake. This study demonstrated that compounds 2, 5-7 were isolated for the first time from this plant and compounds 2 and 7 were isolated for the first time in genus Ligustrum, L. lucidum flowers, KR, KF and HO may possess potential hypoglycemic effect.http://www.sciencedirect.com/science/article/pii/S2213453019302848Ligustrum lucidum flowersChemical composition3T3-L1 adipocytes |
| spellingShingle | Lili Cui Jinmei Wang Mengke Wang Baoguang Wang Zhenhua Liu Jinfeng Wei Wenyi Kang Chemical composition and glucose uptake effect on 3T3-L1 adipocytes of Ligustrum lucidum Ait. flowers Food Science and Human Wellness Ligustrum lucidum flowers Chemical composition 3T3-L1 adipocytes |
| title | Chemical composition and glucose uptake effect on 3T3-L1 adipocytes of Ligustrum lucidum Ait. flowers |
| title_full | Chemical composition and glucose uptake effect on 3T3-L1 adipocytes of Ligustrum lucidum Ait. flowers |
| title_fullStr | Chemical composition and glucose uptake effect on 3T3-L1 adipocytes of Ligustrum lucidum Ait. flowers |
| title_full_unstemmed | Chemical composition and glucose uptake effect on 3T3-L1 adipocytes of Ligustrum lucidum Ait. flowers |
| title_short | Chemical composition and glucose uptake effect on 3T3-L1 adipocytes of Ligustrum lucidum Ait. flowers |
| title_sort | chemical composition and glucose uptake effect on 3t3 l1 adipocytes of ligustrum lucidum ait flowers |
| topic | Ligustrum lucidum flowers Chemical composition 3T3-L1 adipocytes |
| url | http://www.sciencedirect.com/science/article/pii/S2213453019302848 |
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