Metabolomic differences and differential biomarkers in the meat of Jinhua pigs and Duroc×Landrace×Yorkshire pigs

Jinhua (JH) pig, a local breed in China, is valued for its thin skin, fine bones, and tender meat. Compared to the primary commercial pig breed in China, Duroc×Landrace×Yorkshire (DLY) pigs, purebred JH pigs exhibit differences in meat characteristics, sensory parameters, and physicochemical indices...

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Main Authors: Xinjie Wang, Yun Xiang, Xiaojun Zhang, Yi Xiang, Yidan Zhu, Jianhong Zhang, Lu Wang, Yanfang Liu, Huanliang Zhan
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:International Journal of Food Properties
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Online Access:https://www.tandfonline.com/doi/10.1080/10942912.2025.2492344
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author Xinjie Wang
Yun Xiang
Xiaojun Zhang
Yi Xiang
Yidan Zhu
Jianhong Zhang
Lu Wang
Yanfang Liu
Huanliang Zhan
author_facet Xinjie Wang
Yun Xiang
Xiaojun Zhang
Yi Xiang
Yidan Zhu
Jianhong Zhang
Lu Wang
Yanfang Liu
Huanliang Zhan
author_sort Xinjie Wang
collection DOAJ
description Jinhua (JH) pig, a local breed in China, is valued for its thin skin, fine bones, and tender meat. Compared to the primary commercial pig breed in China, Duroc×Landrace×Yorkshire (DLY) pigs, purebred JH pigs exhibit differences in meat characteristics, sensory parameters, and physicochemical indices. To analyze these differences between JH and DLY pork, untargeted metabolomics were employed to identify differential metabolites. 1D-myo-inositol 1,2,3,5,6-pentakisphosphate, N-acetylneuraminic acid, 6-keto-prostaglandin F1alpha, 9(S),12(S),13(S)-TriHOME, uridine-5′-diphosphate, erucic acid, keto-deoxy-nonulonic acid, phenylacetylglycine, and succinic acid were selected as biomarkers of the nonvolatile metabolome, all of which were downregulated in the JH group. Additionally, 3-octen-2-one, 4-methyl-nonane, 1-octen-3-ol, 2,3-butanediol, carvone, and benzeneacetic acid were downregulated in the JH group, except for 5-methyl-4-hexene-1-yl acetate, which was selected as a potential biomarker for the volatile differential metabolome. These biomarkers are involved in energy metabolism, lipid synthesis, stress response mechanisms, and other metabolic pathways. Flavoromics was used to annotate the differential metabolites, revealing that JH pork exhibited a lower abundance of fruity, sweet and green flavor profiles. This study provides fundamental data to enhance our understanding of the flavor and quality characteristics of JH pork and lays a foundation for the development and utilization of JH pig germplasm resources.
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spelling doaj-art-768d0f3d629949fdb7c857f0457a2fd02025-08-20T03:06:44ZengTaylor & Francis GroupInternational Journal of Food Properties1094-29121532-23862025-12-0128110.1080/10942912.2025.2492344Metabolomic differences and differential biomarkers in the meat of Jinhua pigs and Duroc×Landrace×Yorkshire pigsXinjie Wang0Yun Xiang1Xiaojun Zhang2Yi Xiang3Yidan Zhu4Jianhong Zhang5Lu Wang6Yanfang Liu7Huanliang Zhan8Faculty of Agriculture, Jinhua University of Vocational Technology, Jinhua, Zhejiang, ChinaAnimal Husbandry Institute, Jinhua Academy of Agricultural Sciences, Jinhua, Zhejiang, ChinaAnimal Husbandry Institute, Jinhua Academy of Agricultural Sciences, Jinhua, Zhejiang, ChinaNutrition Branch, Jinhua Municipal Central Hospital Medical Group, Jinhua, Zhejiang, ChinaAnimal Husbandry Institute, Jinhua Academy of Agricultural Sciences, Jinhua, Zhejiang, ChinaAnimal Epidemic Prevention and Quarantine Centre, Jinhua Wucheng District Agricultural and Rural Bureau, Jinhua, Zhejiang, ChinaAnimal Epidemic Prevention and Quarantine Centre, Jinhua Wucheng District Agricultural and Rural Bureau, Jinhua, Zhejiang, ChinaAnimal Epidemic Prevention and Quarantine Centre, Jinhua Wucheng District Agricultural and Rural Bureau, Jinhua, Zhejiang, ChinaStrategic Management Department, Jinhua Pig Ecological Ranch, Zhejiang Zhongcai Co, Zhuji, Zhejiang, ChinaJinhua (JH) pig, a local breed in China, is valued for its thin skin, fine bones, and tender meat. Compared to the primary commercial pig breed in China, Duroc×Landrace×Yorkshire (DLY) pigs, purebred JH pigs exhibit differences in meat characteristics, sensory parameters, and physicochemical indices. To analyze these differences between JH and DLY pork, untargeted metabolomics were employed to identify differential metabolites. 1D-myo-inositol 1,2,3,5,6-pentakisphosphate, N-acetylneuraminic acid, 6-keto-prostaglandin F1alpha, 9(S),12(S),13(S)-TriHOME, uridine-5′-diphosphate, erucic acid, keto-deoxy-nonulonic acid, phenylacetylglycine, and succinic acid were selected as biomarkers of the nonvolatile metabolome, all of which were downregulated in the JH group. Additionally, 3-octen-2-one, 4-methyl-nonane, 1-octen-3-ol, 2,3-butanediol, carvone, and benzeneacetic acid were downregulated in the JH group, except for 5-methyl-4-hexene-1-yl acetate, which was selected as a potential biomarker for the volatile differential metabolome. These biomarkers are involved in energy metabolism, lipid synthesis, stress response mechanisms, and other metabolic pathways. Flavoromics was used to annotate the differential metabolites, revealing that JH pork exhibited a lower abundance of fruity, sweet and green flavor profiles. This study provides fundamental data to enhance our understanding of the flavor and quality characteristics of JH pork and lays a foundation for the development and utilization of JH pig germplasm resources.https://www.tandfonline.com/doi/10.1080/10942912.2025.2492344Jinhua pigDuroc×Landrace×Yorkshire pignon-volatile metabolomicsvolatile metabolomicsflavor substance
spellingShingle Xinjie Wang
Yun Xiang
Xiaojun Zhang
Yi Xiang
Yidan Zhu
Jianhong Zhang
Lu Wang
Yanfang Liu
Huanliang Zhan
Metabolomic differences and differential biomarkers in the meat of Jinhua pigs and Duroc×Landrace×Yorkshire pigs
International Journal of Food Properties
Jinhua pig
Duroc×Landrace×Yorkshire pig
non-volatile metabolomics
volatile metabolomics
flavor substance
title Metabolomic differences and differential biomarkers in the meat of Jinhua pigs and Duroc×Landrace×Yorkshire pigs
title_full Metabolomic differences and differential biomarkers in the meat of Jinhua pigs and Duroc×Landrace×Yorkshire pigs
title_fullStr Metabolomic differences and differential biomarkers in the meat of Jinhua pigs and Duroc×Landrace×Yorkshire pigs
title_full_unstemmed Metabolomic differences and differential biomarkers in the meat of Jinhua pigs and Duroc×Landrace×Yorkshire pigs
title_short Metabolomic differences and differential biomarkers in the meat of Jinhua pigs and Duroc×Landrace×Yorkshire pigs
title_sort metabolomic differences and differential biomarkers in the meat of jinhua pigs and duroc landrace yorkshire pigs
topic Jinhua pig
Duroc×Landrace×Yorkshire pig
non-volatile metabolomics
volatile metabolomics
flavor substance
url https://www.tandfonline.com/doi/10.1080/10942912.2025.2492344
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