Crop Productivity, Phytochemicals, and Bioactivities of Wild and Grown in Controlled Environment Slender Amaranth (<i>Amaranthus viridis</i> L.)

<i>Amaranthus viridis</i> L. is a wild edible plant that occasionally is cultivated as an alternative crop because of its interest as a functional food and its adaptation to high-saline soils. In this work, leaves from <i>A. viridis</i> were compared with their grown in contr...

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Main Authors: Tatiana Pagan Loeiro da Cunha-Chiamolera, Tarik Chileh-Chelh, Miguel Urrestarazu, Mohamed Ezzaitouni, Rosalía López-Ruiz, Manuela Gallón-Bedoya, Miguel Á. Rincón-Cervera, José L. Guil-Guerrero
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Language:English
Published: MDPI AG 2024-09-01
Series:Agronomy
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Online Access:https://www.mdpi.com/2073-4395/14/9/2038
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author Tatiana Pagan Loeiro da Cunha-Chiamolera
Tarik Chileh-Chelh
Miguel Urrestarazu
Mohamed Ezzaitouni
Rosalía López-Ruiz
Manuela Gallón-Bedoya
Miguel Á. Rincón-Cervera
José L. Guil-Guerrero
author_facet Tatiana Pagan Loeiro da Cunha-Chiamolera
Tarik Chileh-Chelh
Miguel Urrestarazu
Mohamed Ezzaitouni
Rosalía López-Ruiz
Manuela Gallón-Bedoya
Miguel Á. Rincón-Cervera
José L. Guil-Guerrero
author_sort Tatiana Pagan Loeiro da Cunha-Chiamolera
collection DOAJ
description <i>Amaranthus viridis</i> L. is a wild edible plant that occasionally is cultivated as an alternative crop because of its interest as a functional food and its adaptation to high-saline soils. In this work, leaves from <i>A. viridis</i> were compared with their grown in controlled environment (GCE) counterparts in a soilless system at electrical conductivities (EC) and different light exposures for assessing growth parameters, moisture, total phenolic and total flavonoid content, phenolic compound profiles, vitamin C, antioxidant activity, and antiproliferative activity against the HT–29 human colorectal cancer cell line. The highest biomass production was obtained using EC of 2.5 dS m<sup>−1</sup> and the AP67 Milk LED lamp. Vitamin C in wild samples ranged from 83.1 to 104.9 mg 100 g<sup>−1</sup> fresh weight (fw), and in GCE ones, it ranged from 112.3 to 236.7 mg 100 g<sup>−1</sup> fw. Measured by the DPPH and ABTS assays, the antioxidant activity was higher in wild than in GCE plants: the ranges for wild samples were in the 1.8–4.9 and 2.0–3.9 mmol of Trolox Equivalent (TE) 100 g<sup>−1</sup> dry weight (dw) ranges, and for GCE ones in the 1.3–1.9 and 1.5–2.2 mmol TE 100 g<sup>−1</sup> dw ranges, respectively. As for phenolic compounds, in wild samples, the range was from 14.65 to 22.70 mg 100 g<sup>−1</sup> fw, and these amounts were much higher than those found in their GCE counterparts, in which the range was from 2.58 to 5.95 mg 100 g<sup>−1</sup> fw. In wild plants three compounds, namely trans-<i>p</i>-coumaric acid, isorhamnetin-3-<i>O</i>-glucoside, and nicotiflorin, accounted for more than half of the total quantified phenolic compounds. The MTT assay revealed concentration- and time-dependent inhibitory effects on HT–29 cells for all checked extracts. Cancer cells were less influenced by extracts from GCE plants, which showed higher GI<sub>50</sub> compared to wild plants. This work improves knowledge on the growth parameters, phytochemical profiles, and biological activities of wild and GCE <i>A. viridis</i>.
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spelling doaj-art-76194d3b66f3407684df6e879ed2c4a22025-08-20T01:56:09ZengMDPI AGAgronomy2073-43952024-09-01149203810.3390/agronomy14092038Crop Productivity, Phytochemicals, and Bioactivities of Wild and Grown in Controlled Environment Slender Amaranth (<i>Amaranthus viridis</i> L.)Tatiana Pagan Loeiro da Cunha-Chiamolera0Tarik Chileh-Chelh1Miguel Urrestarazu2Mohamed Ezzaitouni3Rosalía López-Ruiz4Manuela Gallón-Bedoya5Miguel Á. Rincón-Cervera6José L. Guil-Guerrero7Vegetal Production Division, University of Almería, 04120 Almería, SpainFood Technology Division, University of Almería, 04120 Almería, SpainVegetal Production Division, University of Almería, 04120 Almería, SpainFood Technology Division, University of Almería, 04120 Almería, SpainDepartment Chemistry-Physics, Analytical Chemistry of Contaminants, University of Almería, 04120 Almería, SpainDepartment of Agricultural and Food Engineering, Faculty of Agricultural Sciences, National University of Colombia, Medellin Campus, Carrera 65 No. 59A-110, Medellín 050034, ColombiaFood Technology Division, University of Almería, 04120 Almería, SpainFood Technology Division, University of Almería, 04120 Almería, Spain<i>Amaranthus viridis</i> L. is a wild edible plant that occasionally is cultivated as an alternative crop because of its interest as a functional food and its adaptation to high-saline soils. In this work, leaves from <i>A. viridis</i> were compared with their grown in controlled environment (GCE) counterparts in a soilless system at electrical conductivities (EC) and different light exposures for assessing growth parameters, moisture, total phenolic and total flavonoid content, phenolic compound profiles, vitamin C, antioxidant activity, and antiproliferative activity against the HT–29 human colorectal cancer cell line. The highest biomass production was obtained using EC of 2.5 dS m<sup>−1</sup> and the AP67 Milk LED lamp. Vitamin C in wild samples ranged from 83.1 to 104.9 mg 100 g<sup>−1</sup> fresh weight (fw), and in GCE ones, it ranged from 112.3 to 236.7 mg 100 g<sup>−1</sup> fw. Measured by the DPPH and ABTS assays, the antioxidant activity was higher in wild than in GCE plants: the ranges for wild samples were in the 1.8–4.9 and 2.0–3.9 mmol of Trolox Equivalent (TE) 100 g<sup>−1</sup> dry weight (dw) ranges, and for GCE ones in the 1.3–1.9 and 1.5–2.2 mmol TE 100 g<sup>−1</sup> dw ranges, respectively. As for phenolic compounds, in wild samples, the range was from 14.65 to 22.70 mg 100 g<sup>−1</sup> fw, and these amounts were much higher than those found in their GCE counterparts, in which the range was from 2.58 to 5.95 mg 100 g<sup>−1</sup> fw. In wild plants three compounds, namely trans-<i>p</i>-coumaric acid, isorhamnetin-3-<i>O</i>-glucoside, and nicotiflorin, accounted for more than half of the total quantified phenolic compounds. The MTT assay revealed concentration- and time-dependent inhibitory effects on HT–29 cells for all checked extracts. Cancer cells were less influenced by extracts from GCE plants, which showed higher GI<sub>50</sub> compared to wild plants. This work improves knowledge on the growth parameters, phytochemical profiles, and biological activities of wild and GCE <i>A. viridis</i>.https://www.mdpi.com/2073-4395/14/9/2038<i>Amaranthus viridis</i>phenolic compoundsvitamin CantioxidantsHT–29 cell linesoilless culture
spellingShingle Tatiana Pagan Loeiro da Cunha-Chiamolera
Tarik Chileh-Chelh
Miguel Urrestarazu
Mohamed Ezzaitouni
Rosalía López-Ruiz
Manuela Gallón-Bedoya
Miguel Á. Rincón-Cervera
José L. Guil-Guerrero
Crop Productivity, Phytochemicals, and Bioactivities of Wild and Grown in Controlled Environment Slender Amaranth (<i>Amaranthus viridis</i> L.)
Agronomy
<i>Amaranthus viridis</i>
phenolic compounds
vitamin C
antioxidants
HT–29 cell line
soilless culture
title Crop Productivity, Phytochemicals, and Bioactivities of Wild and Grown in Controlled Environment Slender Amaranth (<i>Amaranthus viridis</i> L.)
title_full Crop Productivity, Phytochemicals, and Bioactivities of Wild and Grown in Controlled Environment Slender Amaranth (<i>Amaranthus viridis</i> L.)
title_fullStr Crop Productivity, Phytochemicals, and Bioactivities of Wild and Grown in Controlled Environment Slender Amaranth (<i>Amaranthus viridis</i> L.)
title_full_unstemmed Crop Productivity, Phytochemicals, and Bioactivities of Wild and Grown in Controlled Environment Slender Amaranth (<i>Amaranthus viridis</i> L.)
title_short Crop Productivity, Phytochemicals, and Bioactivities of Wild and Grown in Controlled Environment Slender Amaranth (<i>Amaranthus viridis</i> L.)
title_sort crop productivity phytochemicals and bioactivities of wild and grown in controlled environment slender amaranth i amaranthus viridis i l
topic <i>Amaranthus viridis</i>
phenolic compounds
vitamin C
antioxidants
HT–29 cell line
soilless culture
url https://www.mdpi.com/2073-4395/14/9/2038
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